Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983128520> ?p ?o ?g. }
- W1983128520 endingPage "25" @default.
- W1983128520 startingPage "14" @default.
- W1983128520 abstract "Identifying the sequence pattern in the radiation mutagenesis is essential for understanding the DNA molecular evolution. The present study aimed to establish an in vitro radiation mutagenesis model using naked DNA and to discover the sequence pattern of radiation-induced mutations.Human immunoglobulin heavy-chain variable region gene HVB was chosen as the targeted sequence. DNA molecules dissolved in water were irradiated with gamma rays (25Gy, 1 Gy/min), and four polymerases with different fidelity were used respectively for amplification. Ultra-sensitive next generation sequencing platform “EasyMF” was then employed to detect the mutation rate of all sites. Statistical analysis was performed using SAS9.4 with alpha 0.05.The main result of this study consisted of two parts. One was comparing of the effects of polymerase with different fidelity on ionizing radiation mutagenesis and establishing the in vitro model. Low fidelity DNA polymerases, Taq and Platinum® Taq DNA polymerase, could display the effects of radiation mutagenesis. However, due to the high error rate of Taq DNA polymerase, Platinum® Taq DNA polymerase was more suitable for studying the sequence pattern of radiation mutagenesis. Another was that mutation rates of individual nucleotides G, C and its doublet combination were significantly higher after radiation treatment.Our findings suggest the fidelity of polymerase could affect the radiation mutagenesis. The nucleotides of G, C and their doublet combination showed higher radiation mutability. The sequence analysis of the radiation-induced mutation was helpful for the study of the evolution of DNA molecules under radiation. In addition, the comparisons of the polymerases was helpful to understand the role of the polymerase in the DNA damage repair." @default.
- W1983128520 created "2016-06-24" @default.
- W1983128520 creator A5071063850 @default.
- W1983128520 creator A5078581374 @default.
- W1983128520 date "2000-04-01" @default.
- W1983128520 modified "2023-09-25" @default.
- W1983128520 title "Effects of Ionizing Radiation in Targeted and Nontargeted Cells" @default.
- W1983128520 cites W1506832741 @default.
- W1983128520 cites W1553616550 @default.
- W1983128520 cites W1559821198 @default.
- W1983128520 cites W1595540702 @default.
- W1983128520 cites W1612736060 @default.
- W1983128520 cites W1760745268 @default.
- W1983128520 cites W1862042845 @default.
- W1983128520 cites W189821932 @default.
- W1983128520 cites W1903133958 @default.
- W1983128520 cites W1917341586 @default.
- W1983128520 cites W1952477712 @default.
- W1983128520 cites W1965390636 @default.
- W1983128520 cites W1965620353 @default.
- W1983128520 cites W1966908061 @default.
- W1983128520 cites W1968029748 @default.
- W1983128520 cites W1976966995 @default.
- W1983128520 cites W1977532213 @default.
- W1983128520 cites W1979175943 @default.
- W1983128520 cites W1980284010 @default.
- W1983128520 cites W1980841158 @default.
- W1983128520 cites W1981797176 @default.
- W1983128520 cites W1982540495 @default.
- W1983128520 cites W1982626186 @default.
- W1983128520 cites W1983065531 @default.
- W1983128520 cites W1988502009 @default.
- W1983128520 cites W1993597074 @default.
- W1983128520 cites W1994552302 @default.
- W1983128520 cites W1994628242 @default.
- W1983128520 cites W1997419670 @default.
- W1983128520 cites W1999050438 @default.
- W1983128520 cites W1999320089 @default.
- W1983128520 cites W2001258296 @default.
- W1983128520 cites W2003477293 @default.
- W1983128520 cites W2005150513 @default.
- W1983128520 cites W2005381573 @default.
- W1983128520 cites W2010594589 @default.
- W1983128520 cites W2011002646 @default.
- W1983128520 cites W2016492849 @default.
- W1983128520 cites W2017498643 @default.
- W1983128520 cites W2018328689 @default.
- W1983128520 cites W2019963243 @default.
- W1983128520 cites W2020185180 @default.
- W1983128520 cites W2020631276 @default.
- W1983128520 cites W2022029701 @default.
- W1983128520 cites W2024781204 @default.
- W1983128520 cites W2025263361 @default.
- W1983128520 cites W2028207680 @default.
- W1983128520 cites W2028361915 @default.
- W1983128520 cites W2029630020 @default.
- W1983128520 cites W2032869902 @default.
- W1983128520 cites W2033950216 @default.
- W1983128520 cites W2034602546 @default.
- W1983128520 cites W2036313173 @default.
- W1983128520 cites W2037621441 @default.
- W1983128520 cites W2037670899 @default.
- W1983128520 cites W2040132566 @default.
- W1983128520 cites W2040465809 @default.
- W1983128520 cites W2041221082 @default.
- W1983128520 cites W2042628846 @default.
- W1983128520 cites W2042965746 @default.
- W1983128520 cites W2043218028 @default.
- W1983128520 cites W2044014838 @default.
- W1983128520 cites W2048569323 @default.
- W1983128520 cites W2053422544 @default.
- W1983128520 cites W2054563608 @default.
- W1983128520 cites W2056036386 @default.
- W1983128520 cites W2057951424 @default.
- W1983128520 cites W2058461613 @default.
- W1983128520 cites W2068449224 @default.
- W1983128520 cites W2069607804 @default.
- W1983128520 cites W2070142066 @default.
- W1983128520 cites W2070693959 @default.
- W1983128520 cites W2072081279 @default.
- W1983128520 cites W2072153851 @default.
- W1983128520 cites W2072569792 @default.
- W1983128520 cites W2073079402 @default.
- W1983128520 cites W2073391655 @default.
- W1983128520 cites W2073728593 @default.
- W1983128520 cites W2073946113 @default.
- W1983128520 cites W2077227511 @default.
- W1983128520 cites W2079194714 @default.
- W1983128520 cites W2080125477 @default.
- W1983128520 cites W2080463882 @default.
- W1983128520 cites W2080568589 @default.
- W1983128520 cites W2080795437 @default.
- W1983128520 cites W2084205200 @default.
- W1983128520 cites W2085729612 @default.
- W1983128520 cites W2087493485 @default.
- W1983128520 cites W2087870236 @default.
- W1983128520 cites W2089471296 @default.
- W1983128520 cites W2090898033 @default.