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- W1983132208 abstract "The carbon-13 kinetic isotope effect on the dehydration of HCO3- by bovine carbonic anhydrase has been measured. To accomplish this, bicarbonate was added to a buffer solution at pH 8 containing carbonic anhydrase under conditions where purging of the product CO2 from the solution is rapid. Measurement of the isotopic composition of the purged CO2 as a function of the concentration of carbonic anhydrase permits calculation of the isotope effect on the enzymic reaction. The isotope effect on the dehydration is k12/k13 = 1.0101 +/- 0.0004. This effect is most consistent with a ping-pong mechanism for carbonic anhydrase action, in which proton transfer to or from the enzyme occurs in a step separate from the dehydration step. Substrate and product dissociation steps are at least 2-3-fold faster than the hydration/dehydration step." @default.
- W1983132208 created "2016-06-24" @default.
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- W1983132208 date "1985-09-01" @default.
- W1983132208 modified "2023-09-23" @default.
- W1983132208 title "Carbon isotope effect on dehydration of bicarbonate ion catalyzed by carbonic anhydrase" @default.
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- W1983132208 doi "https://doi.org/10.1021/bi00340a028" @default.
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