FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983181572> ?p ?o ?g. }

• W1983181572 endingPage "453" @default.
• W1983181572 startingPage "447" @default.
• W1983181572 abstract "Human glycine N-acyltransferase (human GLYAT) detoxifies a wide range of endogenous and xenobiotic metabolites, including benzoate and salicylate. Significant inter-individual variation exists in glycine conjugation capacity. The molecular basis for this variability is not known. To investigate the influence of single nucleotide polymorphisms (SNPs) in the GLYAT coding sequence on enzyme activity, we expressed and characterised a recombinant human GLYAT. Site-directed mutagenesis was used to generate six non-synonymous SNP variants of the enzyme (K16N; S17T; R131H; N156S; F168L; R199C). The variants were expressed, purified, and enzymatically characterised. The enzyme activities of the K16N, S17T and R131H variants were similar to that of the wild-type, whereas the N156S variant was more active, the F168L variant less active, and the R199C variant was inactive. We also generated an E227Q mutant, which lacks the catalytic residue proposed by Badenhorst et al. (2012). This mutant was inactive compared to the wild-type recombinant human GLYAT. A molecular model of human GLYAT containing coenzyme A (CoA) was generated which revealed that the inactivity of the R199C variant could be due to the substitution of the highly conserved Arg(199) and destabilisation of an α-loop-α motif which is important for substrate binding in the GNAT superfamily. The finding that SNP variations in the human GLYAT gene influence the kinetic properties of the enzyme may explain some of the inter-individual variation in glycine conjugation capacity, which is relevant to the metabolism of xenobiotics such as aspirin and the industrial solvent xylene, and to the treatment of some metabolic disorders." @default.
• W1983181572 created "2016-06-24" @default.
• W1983181572 creator A5012025850 @default.
• W1983181572 creator A5043877858 @default.
• W1983181572 creator A5081589040 @default.
• W1983181572 creator A5087942494 @default.
• W1983181572 date "2013-02-01" @default.
• W1983181572 modified "2023-09-25" @default.
• W1983181572 title "Characterisation of the influence of genetic variations on the enzyme activity of a recombinant human glycine N-acyltransferase" @default.
• W1983181572 cites W126864726 @default.
• W1983181572 cites W1530639715 @default.
• W1983181572 cites W1547383512 @default.
• W1983181572 cites W1973220508 @default.
• W1983181572 cites W1986941374 @default.
• W1983181572 cites W1990842900 @default.
• W1983181572 cites W1996114410 @default.
• W1983181572 cites W2004711406 @default.
• W1983181572 cites W2006572288 @default.
• W1983181572 cites W2012973022 @default.
• W1983181572 cites W2022658862 @default.
• W1983181572 cites W2026550655 @default.
• W1983181572 cites W2026585629 @default.
• W1983181572 cites W2036874847 @default.
• W1983181572 cites W2038221985 @default.
• W1983181572 cites W2045189096 @default.
• W1983181572 cites W2045298158 @default.
• W1983181572 cites W2056256726 @default.
• W1983181572 cites W2066375788 @default.
• W1983181572 cites W2067209497 @default.
• W1983181572 cites W2069616912 @default.
• W1983181572 cites W2076984373 @default.
• W1983181572 cites W2078871999 @default.
• W1983181572 cites W2086478133 @default.
• W1983181572 cites W2088993157 @default.
• W1983181572 cites W2089716907 @default.
• W1983181572 cites W2100028870 @default.
• W1983181572 cites W2100837269 @default.
• W1983181572 cites W2111199460 @default.
• W1983181572 cites W2112457344 @default.
• W1983181572 cites W2122628215 @default.
• W1983181572 cites W2132629607 @default.
• W1983181572 cites W2135355814 @default.
• W1983181572 cites W2147842931 @default.
• W1983181572 cites W2339614621 @default.
• W1983181572 cites W4232534952 @default.
• W1983181572 doi "https://doi.org/10.1016/j.gene.2012.12.003" @default.
• W1983181572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23237781" @default.
• W1983181572 hasPublicationYear "2013" @default.
• W1983181572 type Work @default.
• W1983181572 sameAs 1983181572 @default.
• W1983181572 citedByCount "19" @default.
• W1983181572 countsByYear W19831815722013 @default.
• W1983181572 countsByYear W19831815722014 @default.
• W1983181572 countsByYear W19831815722015 @default.
• W1983181572 countsByYear W19831815722016 @default.
• W1983181572 countsByYear W19831815722017 @default.
• W1983181572 countsByYear W19831815722018 @default.
• W1983181572 countsByYear W19831815722019 @default.
• W1983181572 countsByYear W19831815722021 @default.
• W1983181572 countsByYear W19831815722023 @default.
• W1983181572 crossrefType "journal-article" @default.
• W1983181572 hasAuthorship W1983181572A5012025850 @default.
• W1983181572 hasAuthorship W1983181572A5043877858 @default.
• W1983181572 hasAuthorship W1983181572A5081589040 @default.
• W1983181572 hasAuthorship W1983181572A5087942494 @default.
• W1983181572 hasConcept C103408237 @default.
• W1983181572 hasConcept C104317684 @default.
• W1983181572 hasConcept C143065580 @default.
• W1983181572 hasConcept C181199279 @default.
• W1983181572 hasConcept C40767141 @default.
• W1983181572 hasConcept C54355233 @default.
• W1983181572 hasConcept C55493867 @default.
• W1983181572 hasConcept C86803240 @default.
• W1983181572 hasConceptScore W1983181572C103408237 @default.
• W1983181572 hasConceptScore W1983181572C104317684 @default.
• W1983181572 hasConceptScore W1983181572C143065580 @default.
• W1983181572 hasConceptScore W1983181572C181199279 @default.
• W1983181572 hasConceptScore W1983181572C40767141 @default.
• W1983181572 hasConceptScore W1983181572C54355233 @default.
• W1983181572 hasConceptScore W1983181572C55493867 @default.
• W1983181572 hasConceptScore W1983181572C86803240 @default.
• W1983181572 hasIssue "2" @default.
• W1983181572 hasLocation W19831815721 @default.
• W1983181572 hasLocation W19831815722 @default.
• W1983181572 hasOpenAccess W1983181572 @default.
• W1983181572 hasPrimaryLocation W19831815721 @default.
• W1983181572 hasRelatedWork W1586318868 @default.
• W1983181572 hasRelatedWork W2006475113 @default.
• W1983181572 hasRelatedWork W2064042198 @default.
• W1983181572 hasRelatedWork W2094237272 @default.
• W1983181572 hasRelatedWork W2102431124 @default.
• W1983181572 hasRelatedWork W2117110650 @default.
• W1983181572 hasRelatedWork W2156674132 @default.
• W1983181572 hasRelatedWork W2169309436 @default.
• W1983181572 hasRelatedWork W258580054 @default.
• W1983181572 hasRelatedWork W2792698313 @default.
• W1983181572 hasVolume "515" @default.
• W1983181572 isParatext "false" @default.

• next