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- W1983189404 abstract "We describe an improved gel-based method with an additive Zn(2+)-cyclen complex (cyclen, 1,4,7,10-tetraazacyclododecane), Zn(2+)-cyclen-PAGE, for mutation detection in DNA fragments by PCR that contain more than 65% Ade/Thy bases and fewer than 100base pairs (bp). Existing techniques have a problem in analyzing such short Ade/Thy-rich fragments because the duplexes are disrupted and are not detectable due to binding of Zn(2+)-cyclen to Thy bases. In this strategy using a PCR primer with a Gua/Cyt-lined sequence attached at its 5'-end, we successfully detected a mutation in an 86-bp Ade/Thy-rich region of the BRCA1 gene from formalin-fixed paraffin-embedded breast cancer-tissue sections." @default.
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- W1983189404 date "2011-01-01" @default.
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- W1983189404 title "Zinc(II)–cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments" @default.
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- W1983189404 doi "https://doi.org/10.1016/j.ab.2010.09.003" @default.
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