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- W1983203401 abstract "ABSTRACT A polymerase chain reaction (PCR)‐based adapter ligation method was developed to determine native barley ( Hordeum vulgare L.) sequences flanking Dissociation ( Ds ) insertions and barley expressed sequence tags (ESTs). This method is simple and efficient, with the majority (∼70%) of queries returning valid sequence information. The success of this method can be traced to experimental protocols that control sample cross contamination and that segregate specific products from nonspecific products. This report describes the protocol in detail, quantifies its efficiency to enable comparisons to future modifications, and discusses specific problems that may be addressed to enable further improvements to this method." @default.
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- W1983203401 date "2012-05-01" @default.
- W1983203401 modified "2023-10-18" @default.
- W1983203401 title "An Efficient Method for Flanking Sequence Isolation in Barley" @default.
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- W1983203401 doi "https://doi.org/10.2135/cropsci2011.10.0560" @default.
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