FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983236475> ?p ?o ?g. }

• W1983236475 abstract "The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are characterized by two fundamental properties: self-renewal and multipotency that allows a stem cell to differentiate into virtually any cell type. The progression stem cell to differentiated cell is characterized by loss of multipotency, structural and morphological changes and the hierarchic activity of transcription factors and signaling molecules, whose activities establish and maintain cell-type specific gene expression patterns. At the molecular level, cell differentiation involves dynamic changes of the structure and composition of chromatin and the detection of those dynamic changes can provide valuable insights into the functional features of stem cells and the cell differentiation process. Chromatin is a highly compacted DNA-protein complex that forms when cells package chromosomal DNA with proteins, mainly histones (4). Stemcellness and cell differentiation has been correlated with the presence of specific arrays of regulatory proteins such as epigenetic factors, histone variants, and transcription factors. Chromatin immunoprecipitation (ChIP) provides a valuable method to monitor the presence of RNA, proteins, and protein modifications in chromatin. The comparison of chromatin from different cell types can elucidate dynamic changes in protein-chromatin associations that occur during cell differentiation. Chromatin immunoprecipitation involves the purification of in vivo cross-linked chromatin. The isolated chromatin is reduced to smaller fragments by enzymatic digestion or mechanical force. Chromatin fragments are precipitated using specific antibodies to target proteins or protein and DNA modifications. The precipitated DNA or RNA is purified and used as a template for PCR or DNA microarray based assays. Prerequisites for a successful ChIP are high quality antibodies to the desired antigen and the availability of chromatin from control cells that do not express the target molecule. ChIP can correlate the presence of proteins, protein and RNA modifications, and RNA with specific target DNA, and depending on the choice of outread tool, detects the association of target molecules at specific target genes or in the context of an entire genome. The comparison of the distribution of proteins in the chromatin of differentiating cells can elucidate the dynamic changes of chromatin composition that coincide with the progression of cells along a cell lineage." @default.
• W1983236475 created "2016-06-24" @default.
• W1983236475 creator A5037324217 @default.
• W1983236475 creator A5043209978 @default.
• W1983236475 creator A5083654967 @default.
• W1983236475 date "2008-07-22" @default.
• W1983236475 modified "2023-10-18" @default.
• W1983236475 title "Chromatin Immunoprecipitation from Human Embryonic Stem Cells" @default.
• W1983236475 cites W1986396604 @default.
• W1983236475 cites W2045899482 @default.
• W1983236475 cites W2046304565 @default.
• W1983236475 cites W2074324848 @default.
• W1983236475 cites W2153424791 @default.
• W1983236475 cites W2158610627 @default.
• W1983236475 doi "https://doi.org/10.3791/780" @default.
• W1983236475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3253610" @default.
• W1983236475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19066517" @default.
• W1983236475 hasPublicationYear "2008" @default.
• W1983236475 type Work @default.
• W1983236475 sameAs 1983236475 @default.
• W1983236475 citedByCount "4" @default.
• W1983236475 countsByYear W19832364752015 @default.
• W1983236475 countsByYear W19832364752020 @default.
• W1983236475 countsByYear W19832364752021 @default.
• W1983236475 crossrefType "journal-article" @default.
• W1983236475 hasAuthorship W1983236475A5037324217 @default.
• W1983236475 hasAuthorship W1983236475A5043209978 @default.
• W1983236475 hasAuthorship W1983236475A5083654967 @default.
• W1983236475 hasBestOaLocation W19832364751 @default.
• W1983236475 hasConcept C101762097 @default.
• W1983236475 hasConcept C104317684 @default.
• W1983236475 hasConcept C134320426 @default.
• W1983236475 hasConcept C137338518 @default.
• W1983236475 hasConcept C145103041 @default.
• W1983236475 hasConcept C148738053 @default.
• W1983236475 hasConcept C150194340 @default.
• W1983236475 hasConcept C167227067 @default.
• W1983236475 hasConcept C176619506 @default.
• W1983236475 hasConcept C191608507 @default.
• W1983236475 hasConcept C28328180 @default.
• W1983236475 hasConcept C41091548 @default.
• W1983236475 hasConcept C54355233 @default.
• W1983236475 hasConcept C552990157 @default.
• W1983236475 hasConcept C56952853 @default.
• W1983236475 hasConcept C64927066 @default.
• W1983236475 hasConcept C67258246 @default.
• W1983236475 hasConcept C83640560 @default.
• W1983236475 hasConcept C86803240 @default.
• W1983236475 hasConcept C95444343 @default.
• W1983236475 hasConceptScore W1983236475C101762097 @default.
• W1983236475 hasConceptScore W1983236475C104317684 @default.
• W1983236475 hasConceptScore W1983236475C134320426 @default.
• W1983236475 hasConceptScore W1983236475C137338518 @default.
• W1983236475 hasConceptScore W1983236475C145103041 @default.
• W1983236475 hasConceptScore W1983236475C148738053 @default.
• W1983236475 hasConceptScore W1983236475C150194340 @default.
• W1983236475 hasConceptScore W1983236475C167227067 @default.
• W1983236475 hasConceptScore W1983236475C176619506 @default.
• W1983236475 hasConceptScore W1983236475C191608507 @default.
• W1983236475 hasConceptScore W1983236475C28328180 @default.
• W1983236475 hasConceptScore W1983236475C41091548 @default.
• W1983236475 hasConceptScore W1983236475C54355233 @default.
• W1983236475 hasConceptScore W1983236475C552990157 @default.
• W1983236475 hasConceptScore W1983236475C56952853 @default.
• W1983236475 hasConceptScore W1983236475C64927066 @default.
• W1983236475 hasConceptScore W1983236475C67258246 @default.
• W1983236475 hasConceptScore W1983236475C83640560 @default.
• W1983236475 hasConceptScore W1983236475C86803240 @default.
• W1983236475 hasConceptScore W1983236475C95444343 @default.
• W1983236475 hasIssue "17" @default.
• W1983236475 hasLocation W19832364751 @default.
• W1983236475 hasLocation W19832364752 @default.
• W1983236475 hasLocation W19832364753 @default.
• W1983236475 hasLocation W19832364754 @default.
• W1983236475 hasOpenAccess W1983236475 @default.
• W1983236475 hasPrimaryLocation W19832364751 @default.
• W1983236475 hasRelatedWork W1449897346 @default.
• W1983236475 hasRelatedWork W1592110616 @default.
• W1983236475 hasRelatedWork W1971291672 @default.
• W1983236475 hasRelatedWork W1983236475 @default.
• W1983236475 hasRelatedWork W1992069167 @default.
• W1983236475 hasRelatedWork W2037920273 @default.
• W1983236475 hasRelatedWork W2114592990 @default.
• W1983236475 hasRelatedWork W2947608130 @default.
• W1983236475 hasRelatedWork W4207009681 @default.
• W1983236475 hasRelatedWork W4313386305 @default.
• W1983236475 isParatext "false" @default.
• W1983236475 isRetracted "false" @default.
• W1983236475 magId "1983236475" @default.
• W1983236475 workType "article" @default.