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• W1983259483 abstract "Abstract. The uptake and accumulation of inorganic carbon has been investigated in Chlorella ellipsoidea cells grown at acid or alkaline pH. Carbonic anhydrase (CA) was detected in ceil extracts but not in intact cells and CA activity in acid-grown cells was considerably less than that in alkali-grown cells. Both cell types demonstrates low K1/2 (CO2) values in the range pH 7.0–8.0 and these were unaffected by O2 concentration. The CO2 compensation concentrations of acid- and alkali-grown cells suspended in aqueous media were not significantly different in the range of pH 6.0–8.0, but at pH 5.0, the CO2 compensation concentrations of acid-grown cells (57.4cm3 m−3) were lower than those of alkali-grown cells (79.2cm3 m−3). The rate of photo-synthetic O2 evolution in the range pH 7.5–8.0 exceeded the calculated rate of CO2 supply two- to three-fold, in both acid- and alkali-grown cells, indicating that HCO3− was taken up by the cells. Accumulation of inorganic carbon was measured at pH 7.5 by silicone-oil centri-fugation, and the concentration of unfixed inorganic carbon was found to be 5.1 mol m−3 in acid-grown and 6.4mol m−3 in alkali-grown cells. These concentrations were 4.6- and 5.9-fold greater than in the external medium. These results indicate that photorespiration is suppressed in both acid- and alkali-grown cells by an intracellular accumulation of inorganic carbon due, in part, to an active uptake of bicarbonate." @default.
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• W1983259483 creator A5045904532 @default.
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• W1983259483 date "1991-05-01" @default.
• W1983259483 modified "2023-10-02" @default.
• W1983259483 title "The acquisition and accumulation of inorganic carbon by the unicellular green alga Chlorella ellipsoidea" @default.
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• W1983259483 doi "https://doi.org/10.1111/j.1365-3040.1991.tb00946.x" @default.
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