Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983353035> ?p ?o ?g. }
- W1983353035 endingPage "1847" @default.
- W1983353035 startingPage "1843" @default.
- W1983353035 abstract "Integration of the Saccharomyces cerevisiae retrotransposon Ty1 into the genome requires Ty1 integrase (IN). Apparent functions of Ty1 IN are target-site determination, cleavage, and joining of donor strands. To further study the mechanism of Ty1 integration, an IN expression plasmid has been constructed for use in yeast. The recombinant IN coding sequence differs from mature Ty1 IN associated with Ty1 virus-like particles only in that it has several additional N-terminal amino acid codons. Inclusion of a polyhistidine tag facilitates purification of recombinant IN by metal chelate chromatography. Recombinant Ty1 IN is active in an in vitro assay with short double-stranded oligonucleotide substrates and has biochemical properties similar to those observed with Ty1 virus-like particles. The full-length Ty1 IN produced in yeast should be useful for further biochemical, genetic, and structural analyses of Ty1 integration and for comparative analyses with retroviral IN proteins." @default.
- W1983353035 created "2016-06-24" @default.
- W1983353035 creator A5076211025 @default.
- W1983353035 creator A5088969123 @default.
- W1983353035 date "1994-03-01" @default.
- W1983353035 modified "2023-09-26" @default.
- W1983353035 title "Expression and partial purification of enzymaticallyactive recombinant Ty1 integrase in Saccharomyces cerevisiae." @default.
- W1983353035 cites W1483936602 @default.
- W1983353035 cites W1570594834 @default.
- W1983353035 cites W1595443666 @default.
- W1983353035 cites W1619075339 @default.
- W1983353035 cites W1621406154 @default.
- W1983353035 cites W1753864123 @default.
- W1983353035 cites W1763828525 @default.
- W1983353035 cites W1819474806 @default.
- W1983353035 cites W1873126638 @default.
- W1983353035 cites W1944392355 @default.
- W1983353035 cites W1952178886 @default.
- W1983353035 cites W1965883561 @default.
- W1983353035 cites W1966815449 @default.
- W1983353035 cites W1973643623 @default.
- W1983353035 cites W1979238913 @default.
- W1983353035 cites W1991149253 @default.
- W1983353035 cites W2000254114 @default.
- W1983353035 cites W2006132678 @default.
- W1983353035 cites W2014265802 @default.
- W1983353035 cites W2017419757 @default.
- W1983353035 cites W2035743113 @default.
- W1983353035 cites W2055995901 @default.
- W1983353035 cites W2059201099 @default.
- W1983353035 cites W2070172523 @default.
- W1983353035 cites W2075336365 @default.
- W1983353035 cites W2086660631 @default.
- W1983353035 cites W2105648514 @default.
- W1983353035 cites W2110661303 @default.
- W1983353035 cites W2117759649 @default.
- W1983353035 cites W2128635872 @default.
- W1983353035 cites W2134887033 @default.
- W1983353035 cites W2139912584 @default.
- W1983353035 cites W2148312059 @default.
- W1983353035 cites W2158733225 @default.
- W1983353035 cites W2171044922 @default.
- W1983353035 doi "https://doi.org/10.1073/pnas.91.5.1843" @default.
- W1983353035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/43260" @default.
- W1983353035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8127892" @default.
- W1983353035 hasPublicationYear "1994" @default.
- W1983353035 type Work @default.
- W1983353035 sameAs 1983353035 @default.
- W1983353035 citedByCount "40" @default.
- W1983353035 countsByYear W19833530352013 @default.
- W1983353035 countsByYear W19833530352015 @default.
- W1983353035 countsByYear W19833530352018 @default.
- W1983353035 countsByYear W19833530352021 @default.
- W1983353035 countsByYear W19833530352022 @default.
- W1983353035 crossrefType "journal-article" @default.
- W1983353035 hasAuthorship W1983353035A5076211025 @default.
- W1983353035 hasAuthorship W1983353035A5088969123 @default.
- W1983353035 hasBestOaLocation W19833530351 @default.
- W1983353035 hasConcept C104317684 @default.
- W1983353035 hasConcept C129312508 @default.
- W1983353035 hasConcept C141231307 @default.
- W1983353035 hasConcept C14430832 @default.
- W1983353035 hasConcept C153911025 @default.
- W1983353035 hasConcept C187882448 @default.
- W1983353035 hasConcept C22744801 @default.
- W1983353035 hasConcept C2777576037 @default.
- W1983353035 hasConcept C2779222958 @default.
- W1983353035 hasConcept C40767141 @default.
- W1983353035 hasConcept C4918238 @default.
- W1983353035 hasConcept C552990157 @default.
- W1983353035 hasConcept C55493867 @default.
- W1983353035 hasConcept C7029365 @default.
- W1983353035 hasConcept C86803240 @default.
- W1983353035 hasConcept C91779695 @default.
- W1983353035 hasConceptScore W1983353035C104317684 @default.
- W1983353035 hasConceptScore W1983353035C129312508 @default.
- W1983353035 hasConceptScore W1983353035C141231307 @default.
- W1983353035 hasConceptScore W1983353035C14430832 @default.
- W1983353035 hasConceptScore W1983353035C153911025 @default.
- W1983353035 hasConceptScore W1983353035C187882448 @default.
- W1983353035 hasConceptScore W1983353035C22744801 @default.
- W1983353035 hasConceptScore W1983353035C2777576037 @default.
- W1983353035 hasConceptScore W1983353035C2779222958 @default.
- W1983353035 hasConceptScore W1983353035C40767141 @default.
- W1983353035 hasConceptScore W1983353035C4918238 @default.
- W1983353035 hasConceptScore W1983353035C552990157 @default.
- W1983353035 hasConceptScore W1983353035C55493867 @default.
- W1983353035 hasConceptScore W1983353035C7029365 @default.
- W1983353035 hasConceptScore W1983353035C86803240 @default.
- W1983353035 hasConceptScore W1983353035C91779695 @default.
- W1983353035 hasIssue "5" @default.
- W1983353035 hasLocation W19833530351 @default.
- W1983353035 hasLocation W19833530352 @default.
- W1983353035 hasLocation W19833530353 @default.
- W1983353035 hasLocation W19833530354 @default.
- W1983353035 hasOpenAccess W1983353035 @default.
- W1983353035 hasPrimaryLocation W19833530351 @default.
- W1983353035 hasRelatedWork W1983353035 @default.