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- W1983375207 abstract "This study was designed to determine the cellular distribution and pattern of expression for the mitochondria-associated protein, prohibitin, during the transitional stages of follicular differentiation within the rat ovary. Immunohistochemical staining techniques were used on frozen sections to examine the localization of prohibitin to preantral, antral, preovulatory, and atretic follicles. Prohibitin localization was also determined in corpus luteum from adult rats, in addition to those from infant and juvenile ovaries, before and after gonadotropin stimulation. Western and Northern blotting techniques were used for qualitative and quantitative assessment of prohibitin expression levels within the ovary. Prohibitin was localized within granulosa cells of infant and juvenile ovaries in a relatively heterogeneous staining pattern. The oocyte also exhibited robust prohibitin expression at all stages of follicular development. In addition, strong prohibitin expression was evident in the corpus luteum as well as in follicles undergoing atresia. Additional data derived from studies involving a GnRH-agonist indicate that increases in prohibitin protein expression correlate with the initial events of apoptosis. Collectively, these results support a growth regulatory role for prohibitin within the rat ovary. Therefore, we propose that prohibitin may serve as an important regulator of granulosa cell fate during follicular development." @default.
- W1983375207 created "2016-06-24" @default.
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- W1983375207 date "1999-09-01" @default.
- W1983375207 modified "2023-10-13" @default.
- W1983375207 title "Immunolocalization and expression of prohibitin, a mitochondrial associated protein within the rat ovaries" @default.
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- W1983375207 doi "https://doi.org/10.1002/(sici)1097-0185(19990901)256:1<40::aid-ar6>3.0.co;2-x" @default.
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