FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983383670> ?p ?o ?g. }

• W1983383670 endingPage "e72409" @default.
• W1983383670 startingPage "e72409" @default.
• W1983383670 abstract "The cell cycle progression in mouse embryonic stem cells (mESCs) is controlled by ion fluxes that alter cell volume [1]. This suggests that ion fluxes might control dynamic changes in morphology over the cell cycle, such as rounding up of the cell at mitosis. However, specific channels regulating such dynamic changes and the possible interactions with actomyosin complex have not been clearly identified. Following RNAseq transcriptome analysis of cell cycle sorted mESCs, we found that expression of the K+ ion channel Erg1 peaked in G1 cell cycle phase, which was confirmed by immunostaining. Inhibition of Erg channel activity caused loss of G1 phase cells via non-apoptotic cell death. Cells first lost the ability of membrane blebbing, a typical feature of cultured embryonic stem cells. Continued Erg inhibition further increased cell volume and the cell eventually ruptured. In addition, atomic force measurements on live cells revealed a decreased cortical stiffness after treatment, suggesting alterations in actomyosin organization. When the intracellular osmotic pressure was experimentally decreased by hypertonic solution or block of K+ ion import via the Na, K-ATPase, cell viability was restored and cells acquired normal volume and blebbing activity. Our results suggest that Erg channels have a critical function in K+ ion homeostasis of mESCs over the cell cycle, and that cell death following Erg inhibition is a consequence of the inability to regulate cell volume." @default.
• W1983383670 created "2016-06-24" @default.
• W1983383670 creator A5005412125 @default.
• W1983383670 creator A5007296262 @default.
• W1983383670 creator A5041007879 @default.
• W1983383670 creator A5042449384 @default.
• W1983383670 creator A5042851935 @default.
• W1983383670 creator A5044751640 @default.
• W1983383670 creator A5047814015 @default.
• W1983383670 creator A5058949558 @default.
• W1983383670 creator A5060601222 @default.
• W1983383670 creator A5066669520 @default.
• W1983383670 creator A5086924433 @default.
• W1983383670 creator A5088849137 @default.
• W1983383670 date "2013-08-02" @default.
• W1983383670 modified "2023-09-30" @default.
• W1983383670 title "Erg Channel Is Critical in Controlling Cell Volume during Cell Cycle in Embryonic Stem Cells" @default.
• W1983383670 cites W1966697925 @default.
• W1983383670 cites W1968272833 @default.
• W1983383670 cites W1975115847 @default.
• W1983383670 cites W2003046273 @default.
• W1983383670 cites W2010898713 @default.
• W1983383670 cites W2011177881 @default.
• W1983383670 cites W2016976019 @default.
• W1983383670 cites W2040371292 @default.
• W1983383670 cites W2043879728 @default.
• W1983383670 cites W2066301717 @default.
• W1983383670 cites W2071418499 @default.
• W1983383670 cites W2080948706 @default.
• W1983383670 cites W2083402331 @default.
• W1983383670 cites W2094009347 @default.
• W1983383670 cites W2101654808 @default.
• W1983383670 cites W2111948630 @default.
• W1983383670 cites W2124985265 @default.
• W1983383670 cites W2126432370 @default.
• W1983383670 cites W2128227827 @default.
• W1983383670 cites W2141927899 @default.
• W1983383670 cites W2142482111 @default.
• W1983383670 cites W2149529722 @default.
• W1983383670 cites W2154431984 @default.
• W1983383670 doi "https://doi.org/10.1371/journal.pone.0072409" @default.
• W1983383670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3732234" @default.
• W1983383670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23936540" @default.
• W1983383670 hasPublicationYear "2013" @default.
• W1983383670 type Work @default.
• W1983383670 sameAs 1983383670 @default.
• W1983383670 citedByCount "5" @default.
• W1983383670 countsByYear W19833836702014 @default.
• W1983383670 countsByYear W19833836702015 @default.
• W1983383670 countsByYear W19833836702019 @default.
• W1983383670 crossrefType "journal-article" @default.
• W1983383670 hasAuthorship W1983383670A5005412125 @default.
• W1983383670 hasAuthorship W1983383670A5007296262 @default.
• W1983383670 hasAuthorship W1983383670A5041007879 @default.
• W1983383670 hasAuthorship W1983383670A5042449384 @default.
• W1983383670 hasAuthorship W1983383670A5042851935 @default.
• W1983383670 hasAuthorship W1983383670A5044751640 @default.
• W1983383670 hasAuthorship W1983383670A5047814015 @default.
• W1983383670 hasAuthorship W1983383670A5058949558 @default.
• W1983383670 hasAuthorship W1983383670A5060601222 @default.
• W1983383670 hasAuthorship W1983383670A5066669520 @default.
• W1983383670 hasAuthorship W1983383670A5086924433 @default.
• W1983383670 hasAuthorship W1983383670A5088849137 @default.
• W1983383670 hasBestOaLocation W19833836701 @default.
• W1983383670 hasConcept C104317684 @default.
• W1983383670 hasConcept C145103041 @default.
• W1983383670 hasConcept C1491633281 @default.
• W1983383670 hasConcept C170493617 @default.
• W1983383670 hasConcept C185592680 @default.
• W1983383670 hasConcept C28328180 @default.
• W1983383670 hasConcept C29537977 @default.
• W1983383670 hasConcept C50254741 @default.
• W1983383670 hasConcept C55493867 @default.
• W1983383670 hasConcept C86803240 @default.
• W1983383670 hasConcept C88254577 @default.
• W1983383670 hasConcept C93304396 @default.
• W1983383670 hasConcept C95444343 @default.
• W1983383670 hasConceptScore W1983383670C104317684 @default.
• W1983383670 hasConceptScore W1983383670C145103041 @default.
• W1983383670 hasConceptScore W1983383670C1491633281 @default.
• W1983383670 hasConceptScore W1983383670C170493617 @default.
• W1983383670 hasConceptScore W1983383670C185592680 @default.
• W1983383670 hasConceptScore W1983383670C28328180 @default.
• W1983383670 hasConceptScore W1983383670C29537977 @default.
• W1983383670 hasConceptScore W1983383670C50254741 @default.
• W1983383670 hasConceptScore W1983383670C55493867 @default.
• W1983383670 hasConceptScore W1983383670C86803240 @default.
• W1983383670 hasConceptScore W1983383670C88254577 @default.
• W1983383670 hasConceptScore W1983383670C93304396 @default.
• W1983383670 hasConceptScore W1983383670C95444343 @default.
• W1983383670 hasIssue "8" @default.
• W1983383670 hasLocation W19833836701 @default.
• W1983383670 hasLocation W19833836702 @default.
• W1983383670 hasLocation W19833836703 @default.
• W1983383670 hasLocation W19833836704 @default.
• W1983383670 hasLocation W19833836705 @default.
• W1983383670 hasLocation W19833836706 @default.
• W1983383670 hasOpenAccess W1983383670 @default.

• next