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- W1983403076 abstract "1. A nuclease which specifically splits the phosphodiester-bond of heat-denatured DNA has been isolated from a product of Aspergillus oryzae. The enzyme has been purified about 1000 times and demonstrated to be more active in the presence of 10−4 M Zn2+ and less active in the presence of an equal amount of RNA. The digestion products from a denatured DNA have been identified as 5′-deoxynucleotides. 2. Using native and heat-denatured DNA's of salmon sperm, as well as the single-stranded DNA of coliphage ФX174, as substrates, the substrate specificity of the enzyme has been studied. A conclusion has been drawn that the enzyme is a denatured DNA-(probably single stranded- DNA-)specific nuclease. 3. The enzyme preparation is associated with ribonuclease activity. It is still in doubt, however, whether this activity belongs to the above-mentioned enzyme protein or not." @default.
- W1983403076 created "2016-06-24" @default.
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- W1983403076 date "1966-01-01" @default.
- W1983403076 modified "2023-10-16" @default.
- W1983403076 title "A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzae" @default.
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- W1983403076 doi "https://doi.org/10.1016/0005-2787(66)90263-2" @default.
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