FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983408093> ?p ?o ?g. }

• W1983408093 endingPage "525" @default.
• W1983408093 startingPage "512" @default.
• W1983408093 abstract "The cell cortex is organized by the dynamic interplay between the plasma membrane, membrane proteins, and the cytoskeleton. Despite the cortical localization of septin heteropolymers in vivo and their direct interaction with phospholipid membranes in vitro, their behavior and roles remain elusive. This study characterizes the major cortical septin assembly found in mammalian tissue culture cells by fluorescence recovery after photobleaching analysis. GFP-tagged septin subunits, which colocalized with cortical actin, exhibited slower turnover than some other cortical proteins that were analyzed (e.g., actin, syntaxin-1A and a glutamate aspartate transporter [GLAST]). Perturbation of actin turnover by cytochalasin D or jasplakinolide retarded the cortical septin turnover, while septin depletion by RNAi did not recognizably affect cortical actin turnover. These phenomena are compatibly interpreted by septins' selective association with a subset of actin-based membrane skeleton, as revealed by rapid-freeze deep-etch immuno-replica electron microscopy. We applied the assay system to test septins' presumptive scaffold function on their physiological binding partners. Septin filament destabilization by RNAi-mediated subunit depletion facilitated the turnover of GLAST, depending on the carboxyl-terminal 29 residues, while a septin filament-stabilizing drug forchlorfenuron restrained more GLAST in the unexchangeable fraction. These data indicate that cortical septin heteropolymers are components of the actin-based membrane skeleton providing scaffolds for their interacting partners probably by impeding their lateral diffusion. We predict that diverse submembranous septin clusters found in vivo may serve as scaffolds or reserve pools for specific membrane-bound proteins. © Wiley-Liss, Inc." @default.
• W1983408093 created "2016-06-24" @default.
• W1983408093 creator A5021449949 @default.
• W1983408093 creator A5023535260 @default.
• W1983408093 creator A5027638642 @default.
• W1983408093 creator A5036125853 @default.
• W1983408093 creator A5059519917 @default.
• W1983408093 creator A5078050165 @default.
• W1983408093 creator A5036747792 @default.
• W1983408093 date "2011-08-25" @default.
• W1983408093 modified "2023-09-27" @default.
• W1983408093 title "Submembranous septins as relatively stable components of actin-based membrane skeleton" @default.
• W1983408093 cites W1483413992 @default.
• W1983408093 cites W1496756449 @default.
• W1983408093 cites W1963768207 @default.
• W1983408093 cites W1969651353 @default.
• W1983408093 cites W1969777343 @default.
• W1983408093 cites W1970218293 @default.
• W1983408093 cites W1973044543 @default.
• W1983408093 cites W1988361359 @default.
• W1983408093 cites W1990032691 @default.
• W1983408093 cites W1991660576 @default.
• W1983408093 cites W1991883398 @default.
• W1983408093 cites W1993434836 @default.
• W1983408093 cites W1996680631 @default.
• W1983408093 cites W2003206650 @default.
• W1983408093 cites W2003688833 @default.
• W1983408093 cites W2009051079 @default.
• W1983408093 cites W2009661553 @default.
• W1983408093 cites W2015410199 @default.
• W1983408093 cites W2016941370 @default.
• W1983408093 cites W2017295930 @default.
• W1983408093 cites W2018159649 @default.
• W1983408093 cites W2030896731 @default.
• W1983408093 cites W2041340250 @default.
• W1983408093 cites W2041483590 @default.
• W1983408093 cites W2051830953 @default.
• W1983408093 cites W2054984177 @default.
• W1983408093 cites W2055211442 @default.
• W1983408093 cites W2055376777 @default.
• W1983408093 cites W2057547625 @default.
• W1983408093 cites W2062840905 @default.
• W1983408093 cites W2069616555 @default.
• W1983408093 cites W2070520948 @default.
• W1983408093 cites W2078116784 @default.
• W1983408093 cites W2078816010 @default.
• W1983408093 cites W2080228464 @default.
• W1983408093 cites W2080874634 @default.
• W1983408093 cites W2087351063 @default.
• W1983408093 cites W2089610893 @default.
• W1983408093 cites W2091168806 @default.
• W1983408093 cites W2093325895 @default.
• W1983408093 cites W2098616171 @default.
• W1983408093 cites W2103265939 @default.
• W1983408093 cites W2105346492 @default.
• W1983408093 cites W2112597950 @default.
• W1983408093 cites W2112967030 @default.
• W1983408093 cites W2113695502 @default.
• W1983408093 cites W2114656957 @default.
• W1983408093 cites W2115265985 @default.
• W1983408093 cites W2117333689 @default.
• W1983408093 cites W2124779290 @default.
• W1983408093 cites W2127398373 @default.
• W1983408093 cites W2128744963 @default.
• W1983408093 cites W2129759722 @default.
• W1983408093 cites W2141169141 @default.
• W1983408093 cites W2143351999 @default.
• W1983408093 cites W2143416847 @default.
• W1983408093 cites W2146200179 @default.
• W1983408093 cites W2149059941 @default.
• W1983408093 cites W2159229369 @default.
• W1983408093 cites W2162634069 @default.
• W1983408093 doi "https://doi.org/10.1002/cm.20528" @default.
• W1983408093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21800439" @default.
• W1983408093 hasPublicationYear "2011" @default.
• W1983408093 type Work @default.
• W1983408093 sameAs 1983408093 @default.
• W1983408093 citedByCount "62" @default.
• W1983408093 countsByYear W19834080932012 @default.
• W1983408093 countsByYear W19834080932013 @default.
• W1983408093 countsByYear W19834080932014 @default.
• W1983408093 countsByYear W19834080932015 @default.
• W1983408093 countsByYear W19834080932016 @default.
• W1983408093 countsByYear W19834080932017 @default.
• W1983408093 countsByYear W19834080932018 @default.
• W1983408093 countsByYear W19834080932019 @default.
• W1983408093 countsByYear W19834080932020 @default.
• W1983408093 countsByYear W19834080932021 @default.
• W1983408093 countsByYear W19834080932022 @default.
• W1983408093 countsByYear W19834080932023 @default.
• W1983408093 crossrefType "journal-article" @default.
• W1983408093 hasAuthorship W1983408093A5021449949 @default.
• W1983408093 hasAuthorship W1983408093A5023535260 @default.
• W1983408093 hasAuthorship W1983408093A5027638642 @default.
• W1983408093 hasAuthorship W1983408093A5036125853 @default.
• W1983408093 hasAuthorship W1983408093A5036747792 @default.
• W1983408093 hasAuthorship W1983408093A5059519917 @default.
• W1983408093 hasAuthorship W1983408093A5078050165 @default.

• next