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• W1983434946 abstract "We have studied the regulation of mouse mammary tumor virus (MMTV) gene expression by measuring the production of virus and the transcription of viral genes in cultured cells. To measure virus production, we have used a new assay based on the hybridization of labeled virus-specific DNA (cDNA) with RNA extracted from virus released into the growth medium. The assay is highly specific and more sensitive than other commonly used methods for measuring MMTV production. We have also measured the levels of virus-specific RNA in various cells by annealing MMTV cDNA with cell RNA. In cell lines and primary explants derived from mammary tumors of several strains of mice the amount of virus production correlates with the level of virus-specific RNA. This suggests that in these cells, transcriptional controls are of primary importance in regulating the production of MMTV. Experiments with dexamethasone (a synthetic glucocorticoid) support this notion since cells treated with the hormone show parallel increases in virus production and intracellular virus-specific RNA. In contrast, a lymphoma cell line (S49) derived from a lymphoma induced by mineral oil in a BALBc mouse contains large quantities of MMTV-specific RNA yet produces extremely low levels of virus. In these cells, mechanisms other than transcriptional controls may be important in regulating virus production." @default.
• W1983434946 created "2016-06-24" @default.
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• W1983434946 date "1975-05-01" @default.
• W1983434946 modified "2023-10-14" @default.
• W1983434946 title "Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: Assay by molecular hybridization" @default.
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• W1983434946 doi "https://doi.org/10.1016/0042-6822(75)90014-8" @default.
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