Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983482678> ?p ?o ?g. }
Showing items 1 to 50 of
50
with 100 items per page.
- W1983482678 abstract "We have compared (+/-)-7r,8t-dihydroxy-9t,10t-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I) binding to three vertebrate histones H2A with different C-terminal regions. HPLC analyses of core histones prepared from nuclei exposed to [3H]BPDE-I showed that rat liver and chicken erythrocyte histones H2A were heavily labeled by [3H]BPDE-I, but Xenopus laevis liver histone H2A was not. This result was confirmed by HPLC analyses of V8-protease digests of BPDE-I bound to histone H2A purified from the three different nuclei. There are significant amino acid sequence differences only in the C-terminal regions of the different histones H2A, where rat liver and chicken erythrocyte histones H2A contain two and one histidine residues, respectively, while the amino acid sequence of Xenopus histone H2A contains no histidine. Pre-treatment of the in situ BPDE-I-modified H2A.2 from rat liver with carboxypeptidase B, which should remove the C-terminal lysine from the protein, resulted in increased retention times on reverse-phase HPLC for the adduct-containing peptides upon subsequent V8-protease digestion. This suggested that the site(s) of BPDE-I modification are located primarily in the C-terminal octapeptide of rat H2A.2. To confirm this, C-terminal V8-peptides of the different histones H2A were isolated and reacted with BPDE-I at physiological pH in vitro. The HPLC analyses of the reaction mixtures indicated that the C-terminal peptide of rat liver and chicken erythrocyte histones H2A was a target site for BPDE-I binding in nuclei. It is suggested that the nucleophilic target amino acid for BPDE-I binding in histone H2A may be a histidine located close to the C terminus." @default.
- W1983482678 created "2016-06-24" @default.
- W1983482678 creator A5068827460 @default.
- W1983482678 creator A5085709435 @default.
- W1983482678 date "1988-01-01" @default.
- W1983482678 modified "2023-09-24" @default.
- W1983482678 title "Comparison of benzo [a]pyrene-diol-epoxide binding to histone H2A with different carboxy-terminal regions" @default.
- W1983482678 doi "https://doi.org/10.1093/carcin/9.3.419" @default.
- W1983482678 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3125994" @default.
- W1983482678 hasPublicationYear "1988" @default.
- W1983482678 type Work @default.
- W1983482678 sameAs 1983482678 @default.
- W1983482678 citedByCount "2" @default.
- W1983482678 crossrefType "journal-article" @default.
- W1983482678 hasAuthorship W1983482678A5068827460 @default.
- W1983482678 hasAuthorship W1983482678A5085709435 @default.
- W1983482678 hasConcept C114246631 @default.
- W1983482678 hasConcept C153911025 @default.
- W1983482678 hasConcept C185592680 @default.
- W1983482678 hasConcept C2777538855 @default.
- W1983482678 hasConcept C552990157 @default.
- W1983482678 hasConcept C55493867 @default.
- W1983482678 hasConcept C64927066 @default.
- W1983482678 hasConcept C86803240 @default.
- W1983482678 hasConceptScore W1983482678C114246631 @default.
- W1983482678 hasConceptScore W1983482678C153911025 @default.
- W1983482678 hasConceptScore W1983482678C185592680 @default.
- W1983482678 hasConceptScore W1983482678C2777538855 @default.
- W1983482678 hasConceptScore W1983482678C552990157 @default.
- W1983482678 hasConceptScore W1983482678C55493867 @default.
- W1983482678 hasConceptScore W1983482678C64927066 @default.
- W1983482678 hasConceptScore W1983482678C86803240 @default.
- W1983482678 hasLocation W19834826781 @default.
- W1983482678 hasLocation W19834826782 @default.
- W1983482678 hasOpenAccess W1983482678 @default.
- W1983482678 hasPrimaryLocation W19834826781 @default.
- W1983482678 hasRelatedWork W1535941896 @default.
- W1983482678 hasRelatedWork W1972401330 @default.
- W1983482678 hasRelatedWork W1984804065 @default.
- W1983482678 hasRelatedWork W1987822850 @default.
- W1983482678 hasRelatedWork W1993431290 @default.
- W1983482678 hasRelatedWork W2022270688 @default.
- W1983482678 hasRelatedWork W2322284682 @default.
- W1983482678 hasRelatedWork W2917304564 @default.
- W1983482678 hasRelatedWork W3198590948 @default.
- W1983482678 hasRelatedWork W4206027097 @default.
- W1983482678 isParatext "false" @default.
- W1983482678 isRetracted "false" @default.
- W1983482678 magId "1983482678" @default.
- W1983482678 workType "article" @default.