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• W1983500426 abstract "RATIONALE: Ferric iron (Fe3+) and phenolic compounds have been shown to bind to peanut allergens. An easy way to remove peanut allergens from peanut extracts is by use of magnetic beads attached with or without phenolics to capture peanut allergens or allergen-Fe3+ complexes.METHODS: Magnetic beads covalently attached with chlorogenic acid (CHL) (a phenolic) were prepared by rotating magnetic aminoethyl beads with a mixture of carbodiimide and CHL. The CHL-beads were washed and treated with a peanut extract. Magnetic beads without CHL were treated with another peanut extract that had been incubated with Fe3+. Protein/allergens that bound to the beads were retrieved on a magetic device by regenerating the beads with 1 M NaCl. Samples including treated extracts were analyzed by SDS-PAGE and ELISA (IgE detection).RESULTS: SDS-PAGE showed that peanut extracts treated with magnetic CHL-beads exhibited a marked reduction in the levels of major peanut allergens and a small reduction in other proteins. IgE bindings in ELISA were reduced as well. Similar observations were made with Fe3+-extracts treated with magnetic plain beads. Protein samples retrieved from regeneration of magnetic beads displayed high levels of major peanut allergens in SDS-PAGE, indicating that the allergens were bound to the beads during treatment.CONCLUSIONS: Magnetic beads covalently attached with CHL captured major peanut allergens and small amounts of other proteins from a peanut extract. Also, peanut allergens, when complexed with Fe3+ in an extract, were captured by magnetic plain beads. Both methods resulted in reduced levels of peanut allergens and IgE binding. RATIONALE: Ferric iron (Fe3+) and phenolic compounds have been shown to bind to peanut allergens. An easy way to remove peanut allergens from peanut extracts is by use of magnetic beads attached with or without phenolics to capture peanut allergens or allergen-Fe3+ complexes. METHODS: Magnetic beads covalently attached with chlorogenic acid (CHL) (a phenolic) were prepared by rotating magnetic aminoethyl beads with a mixture of carbodiimide and CHL. The CHL-beads were washed and treated with a peanut extract. Magnetic beads without CHL were treated with another peanut extract that had been incubated with Fe3+. Protein/allergens that bound to the beads were retrieved on a magetic device by regenerating the beads with 1 M NaCl. Samples including treated extracts were analyzed by SDS-PAGE and ELISA (IgE detection). RESULTS: SDS-PAGE showed that peanut extracts treated with magnetic CHL-beads exhibited a marked reduction in the levels of major peanut allergens and a small reduction in other proteins. IgE bindings in ELISA were reduced as well. Similar observations were made with Fe3+-extracts treated with magnetic plain beads. Protein samples retrieved from regeneration of magnetic beads displayed high levels of major peanut allergens in SDS-PAGE, indicating that the allergens were bound to the beads during treatment. CONCLUSIONS: Magnetic beads covalently attached with CHL captured major peanut allergens and small amounts of other proteins from a peanut extract. Also, peanut allergens, when complexed with Fe3+ in an extract, were captured by magnetic plain beads. Both methods resulted in reduced levels of peanut allergens and IgE binding." @default.
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• W1983500426 date "2010-02-01" @default.
• W1983500426 modified "2023-09-25" @default.
• W1983500426 title "Using Magnetic Beads to Reduce Peanut Allergens from Peanut Extracts" @default.
• W1983500426 doi "https://doi.org/10.1016/j.jaci.2009.12.873" @default.
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