Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983519686> ?p ?o ?g. }
- W1983519686 endingPage "102" @default.
- W1983519686 startingPage "95" @default.
- W1983519686 abstract "We describe the evaluation of doxorubicin-loaded PEG–PE micelles targeting using an ovarian cancer cell spheroid model. Most ovarian cancer patients present at an advanced clinical stage and develop resistance to standard of care platinum/taxane therapy. Doxorubicin is also approved for ovarian cancer but had limited benefits in refractory patients. In this study, we used drug-resistant spheroid cultures of ovarian carcinoma to evaluate the uptake and cytotoxicity of an antibody-targeted doxorubicin formulation. Doxorubicin was encapsulated in polyethylene glycol–phosphatidyl ethanolamine (PEG–PE) conjugated micelles. The doxorubicin-loaded PEG–PE micelles (MDOX) were further decorated with a cancer cell-specific monoclonal 2C5 antibody to obtain doxorubicin-loaded immunomicelles (2C5-MDOX). Targeting and resulting toxicity of doxorubicin-loaded PEG–PE micelles were evaluated in three dimensional cancer cell spheroids. Superior accumulation of 2C5-MDOX compared to free doxorubicin or untargeted MDOX in spheroids was evidenced both by flow cytometry, fluorescence and confocal microscopy. Interestingly, even higher toxicity was measured by lactate dehydrogenase release and terminal deoxynucleotidyl transferase dUTP nick end labeling of targeted doxorubicin micelles in Bcl-2 overexpressing adriamycin-resistant spheroids. Overall, these results support use of spheroids to evaluate tumor targeted drug delivery." @default.
- W1983519686 created "2016-06-24" @default.
- W1983519686 creator A5028548558 @default.
- W1983519686 creator A5084391530 @default.
- W1983519686 creator A5087701563 @default.
- W1983519686 date "2012-11-01" @default.
- W1983519686 modified "2023-10-18" @default.
- W1983519686 title "Accumulation and toxicity of antibody-targeted doxorubicin-loaded PEG–PE micelles in ovarian cancer cell spheroid model" @default.
- W1983519686 cites W1523517678 @default.
- W1983519686 cites W1593844282 @default.
- W1983519686 cites W1892647714 @default.
- W1983519686 cites W1964553162 @default.
- W1983519686 cites W1967293289 @default.
- W1983519686 cites W1970239046 @default.
- W1983519686 cites W1975221708 @default.
- W1983519686 cites W1989109875 @default.
- W1983519686 cites W1989230986 @default.
- W1983519686 cites W1995637639 @default.
- W1983519686 cites W2003837018 @default.
- W1983519686 cites W2005655958 @default.
- W1983519686 cites W2005791230 @default.
- W1983519686 cites W2005840555 @default.
- W1983519686 cites W2006147855 @default.
- W1983519686 cites W2006165138 @default.
- W1983519686 cites W2008171455 @default.
- W1983519686 cites W2008290615 @default.
- W1983519686 cites W2015164514 @default.
- W1983519686 cites W2019633596 @default.
- W1983519686 cites W2020830322 @default.
- W1983519686 cites W2026438770 @default.
- W1983519686 cites W2028392530 @default.
- W1983519686 cites W2029983315 @default.
- W1983519686 cites W2031803403 @default.
- W1983519686 cites W2033474014 @default.
- W1983519686 cites W2034387263 @default.
- W1983519686 cites W2044421413 @default.
- W1983519686 cites W2047311008 @default.
- W1983519686 cites W2052606768 @default.
- W1983519686 cites W2055483349 @default.
- W1983519686 cites W2055904567 @default.
- W1983519686 cites W2055951605 @default.
- W1983519686 cites W2056347214 @default.
- W1983519686 cites W2059485990 @default.
- W1983519686 cites W2063396729 @default.
- W1983519686 cites W2070743700 @default.
- W1983519686 cites W2071532342 @default.
- W1983519686 cites W2075889966 @default.
- W1983519686 cites W2082246802 @default.
- W1983519686 cites W2083710155 @default.
- W1983519686 cites W2084517535 @default.
- W1983519686 cites W2085170203 @default.
- W1983519686 cites W2087094217 @default.
- W1983519686 cites W2087597184 @default.
- W1983519686 cites W2092706112 @default.
- W1983519686 cites W2093079379 @default.
- W1983519686 cites W2102897174 @default.
- W1983519686 cites W2104197204 @default.
- W1983519686 cites W2105017561 @default.
- W1983519686 cites W2105547623 @default.
- W1983519686 cites W2110716203 @default.
- W1983519686 cites W2116402747 @default.
- W1983519686 cites W2117675993 @default.
- W1983519686 cites W2132063042 @default.
- W1983519686 cites W2133440826 @default.
- W1983519686 cites W2138035969 @default.
- W1983519686 cites W2147478966 @default.
- W1983519686 cites W2154885792 @default.
- W1983519686 cites W2155035501 @default.
- W1983519686 cites W2158743877 @default.
- W1983519686 cites W2168278139 @default.
- W1983519686 cites W2170779986 @default.
- W1983519686 cites W2171384411 @default.
- W1983519686 cites W2212287095 @default.
- W1983519686 cites W4211147776 @default.
- W1983519686 doi "https://doi.org/10.1016/j.jconrel.2012.09.003" @default.
- W1983519686 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3502646" @default.
- W1983519686 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22974689" @default.
- W1983519686 hasPublicationYear "2012" @default.
- W1983519686 type Work @default.
- W1983519686 sameAs 1983519686 @default.
- W1983519686 citedByCount "118" @default.
- W1983519686 countsByYear W19835196862013 @default.
- W1983519686 countsByYear W19835196862014 @default.
- W1983519686 countsByYear W19835196862015 @default.
- W1983519686 countsByYear W19835196862016 @default.
- W1983519686 countsByYear W19835196862017 @default.
- W1983519686 countsByYear W19835196862018 @default.
- W1983519686 countsByYear W19835196862019 @default.
- W1983519686 countsByYear W19835196862020 @default.
- W1983519686 countsByYear W19835196862021 @default.
- W1983519686 countsByYear W19835196862022 @default.
- W1983519686 countsByYear W19835196862023 @default.
- W1983519686 crossrefType "journal-article" @default.
- W1983519686 hasAuthorship W1983519686A5028548558 @default.
- W1983519686 hasAuthorship W1983519686A5084391530 @default.
- W1983519686 hasAuthorship W1983519686A5087701563 @default.
- W1983519686 hasBestOaLocation W19835196862 @default.
- W1983519686 hasConcept C11268172 @default.