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- W1983554468 abstract "► The study aimed at improvement of the sensitivity of influenza A antigen detection by detecting the three most abundant influenza A viral proteins present in influenza A infected cells. ► Monoclonal antibody pairs for detecting the nucleoprotein (NP), matrix (M), and NS1 proteins were selected. Each MAb pair was used for the assay optimization for the sandwich ELISA. ► Combined NP, M, and NS1 antigen detection improved the assay sensitivity significantly as compared to the individual antigen detection. A new modified triple-antigen detection test was developed for the direct detection of the influenza A virus. The nucleoprotein (NP), matrix (M), and non-structural (NS1) proteins were used as target antigens because they are abundant in infected cells. Monoclonal antibodies specific to the NP, M, and NS1 proteins were generated. The antibody pairs were selected and evaluated for their reactivity individually and in combination in the triple-antigen detection using sandwich ELISA. Triple-antigen detection demonstrated a higher sensitivity than individual antigen detection when tested with both the H1N1 and H3N2 influenza A viruses. This was illustrated by the 4-fold lower limit of detection of the triple-antigen test than the individual antigen detection test. The findings demonstrated that the sensitivity of influenza A antigen detection was improved with the triple-antigen detection system as compared to individual antigen detection. Therefore, this technique could be a useful tool for the direct detection of cell-associated influenza A antigen. Furthermore, it could provide a basis for the development of a rapid triple-antigen test for influenza A diagnosis." @default.
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- W1983554468 date "2012-10-01" @default.
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- W1983554468 title "Improved sensitivity of influenza A antigen detection using a combined NP, M, and NS1 sandwich ELISA" @default.
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- W1983554468 doi "https://doi.org/10.1016/j.jviromet.2012.05.021" @default.
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