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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983621817> ?p ?o ?g. }

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• W1983621817 startingPage "10340" @default.
• W1983621817 abstract "We describe an assay for in vivo protein interactions. Protein fusions containing ubiquitin, a 76-residue, single-domain protein, are rapidly cleaved in vivo by ubiquitin-specific proteases, which recognize the folded conformation of ubiquitin. When a C-terminal fragment of ubiquitin (C(ub)) is expressed as a fusion to a reporter protein, the fusion is cleaved only if an N-terminal fragment of ubiquitin (Nub) is also expressed in the same cell. This reconstitution of native ubiquitin from its fragments, detectable by the in vivo cleavage assay, is not observed with a mutationally altered Nub. However, if C(ub) and the altered Nub are each linked to polypeptides that interact in vivo, the cleavage of the fusion containing C(ub) is restored, yielding a generally applicable assay for kinetic and equilibrium aspects of in vivo protein interactions. This method, termed USPS (ubiquitin-based split-protein sensor), makes it possible to monitor a protein-protein interaction as a function of time, at the natural sites of this interaction in a living cell." @default.
• W1983621817 created "2016-06-24" @default.
• W1983621817 creator A5039562263 @default.
• W1983621817 creator A5058515611 @default.
• W1983621817 date "1994-10-25" @default.
• W1983621817 modified "2023-10-18" @default.
• W1983621817 title "Split ubiquitin as a sensor of protein interactions in vivo." @default.
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• W1983621817 doi "https://doi.org/10.1073/pnas.91.22.10340" @default.
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