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• W1983623512 abstract "Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. Elucidation of the major antigenic epitopes of swHEV is essential for the effective control of swHEV epidemics.By bioinformatic analysis, we identified and then synthesized 12 peptides from open reading frames (ORFs) ORF1, ORF2 and ORF3, including swHEV-1 - swHEV-12. Using the results from ELISA, we selected swHEV-11 as the best candidate antigen and used it as a coating antigen for the development of peptide-based swine anti-HEV ELISA kits. The coefficient of variation (CV) the coefficient of variation (CV) varied between 4.3-7.2% in the same batch, and between 8.2-17.7% in six different batches. When comparing our swine peptide-based kit with the commercial recombinant-based kit, the humane anti-HEV IgG test had a 73.4% correspondence rate for them.This is the first systemic study to screen the diagnostic peptides of swHEV and our findings strongly suggest that peptide swHEV-11 is a potent diagnostic reagent of swHEV that could be used in the development of highly efficient diagnostic assays for the specific and highly sensitive detection of anti-HEV activity in swine serum samples." @default.
• W1983623512 created "2016-06-24" @default.
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• W1983623512 date "2009-11-04" @default.
• W1983623512 modified "2023-09-26" @default.
• W1983623512 title "Screening of specific diagnostic peptides of swine hepatitis E virus" @default.
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• W1983623512 doi "https://doi.org/10.1186/1743-422x-6-186" @default.
• W1983623512 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2780410" @default.
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