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• W1983642474 abstract "Objective: To study activation of blood neutrophils in subjects after in vitro and in vivo stimulation. Methods: Blood from healthy volunteers was collected before and after their exposure to organic dust in a pig farm. Surface markers of neutrophil activation were analyzed by flow cytometry. Results: In vivo, there was a diurnal variation in basal levels of CD11b. Swine dust exposure increased the number of blood neutrophils and the levels of myeloperoxidase without effects on CD11b or Cys-X-Cys receptor 1 (CXCR-1) expression. However, a positive correlation between changes of myeloperoxidase and CD11b expression was observed. In vitro, N-formyl-methionyl-leucyl-phenylalanine (fMLP), lipopolysaccharide (LPS), leukotriene (LT)B4, LTD4, and swine dust stimulated the expression of CD11b. fMLP, LPS, and LTB4 diminished the expression of CXCR-1. Conclusions: Exposure to swine dust in vitro activated neutrophils and inhalation of swine dust caused neutrophilia." @default.
• W1983642474 created "2016-06-24" @default.
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• W1983642474 date "2007-02-01" @default.
• W1983642474 modified "2023-10-03" @default.
• W1983642474 title "Differential Pattern of Human Blood Neutrophil Activation After Stimulation With Organic Dust in Vitro and in Vivo" @default.
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• W1983642474 doi "https://doi.org/10.1097/jom.0b013e31802f3c4a" @default.
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