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- W1983647670 abstract "Transfer ribonucleic acid from baker's yeast was fractionated by means of DEAE-Sephadex column chromatography. The histidine-specific tRNA-rich fraction thus obtained was further purified by reversed-phase column chromatography, by means of which histidine-specific tRNA was separated into two components. The major component of the two, designated as histidine-specific tRNA I, was isolated in high purity. From the amino acid-acceptor activity and several other criteria, it has been concluded that histidine-specific tRNA I thus obtained is sufficiently pure to use for structural study. The histidine-specific tRNA I as mentioned above did not accept histidine with Escherichia coli aminoacyl-tRNA synthetase, while histidine-specific tRNA II, a minor component, did." @default.
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- W1983647670 date "1967-10-01" @default.
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- W1983647670 title "Purification of histidine-specific transfer RNA from yeast" @default.
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- W1983647670 doi "https://doi.org/10.1016/0005-2787(67)90119-0" @default.
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