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- W1983670861 abstract "To apply the latex agglutination lectin assay (LALA) to carbohydrate ligands, monosaccharide derivatives were incorporated onto latex beads by various methods, and the usefulness of the resulting beads was evaluated. The best outcome, which resulted in aggregation with lectin concentrations of 1 to 4 μg/mL, was obtained when latex beads coated with bovine serum albumin were treated with divinylsulfone, a linker agent, and then with 2-aminoethyl glycosides. Monosaccharides with an amino or anomeric hydroxyl group other than N-acetylglucosamine were applicable in this direct LALA. For example, mannose- and 5-thiomannose-coupled latex beads showed aggregation with minimum concanavalin (ConA) concentrations of 4 and 32 μg/mL, respectively. An inhibition assay was more versatile than the direct LALA, and the standardized inhibition activity (EC0 50) was determined for several compounds. Representative EC0 50 data for mannose, methyl mannoside, and p-nitrophenyl mannoside (1, 0.12, and 0.06 mM, respectively) ar..." @default.
- W1983670861 created "2016-06-24" @default.
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- W1983670861 date "2009-02-21" @default.
- W1983670861 modified "2023-09-24" @default.
- W1983670861 title "A Facile Latex Agglutination Lectin Assay (LALA) for Weakly Binding Ligands" @default.
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- W1983670861 doi "https://doi.org/10.1080/07328300802696207" @default.
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