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• W1983687265 abstract "The response of anthers to in vitro culture and effect of growth regulators, temperature pretreatment of anthers have been studied in Calypso and Green Long cultivars of cucumber (Cucumis sativus L.). Direct and callus mediated embryogenesis (embryogenic calli with embryos) was induced in Calypso and Green Long cultivars respectively on B5 medium (Gamborg’s medium) supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and α-naphthaleneacetic acid (NAA) alone as well as 2,4-D in combination with N6-benzylaminopurine/kinetin/thidiazuron (BAP/KN/TDZ). Optimal embryogenic calli/embryos were induced on B5 medium supplemented with 2.0 μM 2,4-D and 1.0 μM BAP. Temperature pretreatment of flower buds at 4 °C for 0–10 days and also at 32 °C for 1 day were tested and best response was obtained at 4 °C for 2 days. Embryo differentiation was achieved on B5 medium containing 0.09 M sucrose, 0.25 μM NAA and 0.25 μM KN. Embryo maturation was on B5 medium supplemented with 5.0 μM abscisic acid (ABA). Embryos germinated into plantlets on B5 medium containing 0.09 M sucrose. Plantlets were acclimatized in the controlled environment. In each cultivars, the root tips of 24 regenerated plantlets were analyzed for ploidy level, of which 21 and 17 have been haploids in Calypso and Green Long, respectively." @default.
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• W1983687265 date "2003-05-01" @default.
• W1983687265 modified "2023-09-30" @default.
• W1983687265 title "Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L." @default.
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• W1983687265 doi "https://doi.org/10.1016/s0304-4238(03)00003-7" @default.
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