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- W1983772767 abstract "Summary Silver staining was used to detect amplified microsatellite loci from the bovine genome. The standard silver‐staining procedure was modified to allow more direct control in the development of signals. Microsatellites were analysed, to compare the silver‐staining technique and the direct incorporation of a radioactive tracer. With silver staining, levels of sensitivity were achieved that are typical of those with radioisotopic detection. Also, silver staining is a cheaper process, is less hazardous to operators and the environment, and can be performed in laboratories without radioisotopic facilities. A significant advantage of silver staining is the speed of the process, producing results of a typing in 1 day. Zusammenfassung Charakterisierung von Rinder Mikrosatelliten durch Silberfärbung Mit Silverstaining (Silberfärbung) wurden amplifizierte Mikrosatellite von Rindern verschiedener Rassen charaktisiert. Für drei Mikrosatelliten wurden einige Nanogramme genomische DNA amplifiziert. Diese Technik erwies sich als empfindlicher und einfacher als die radioisotopische Methodologie und ist auch billiger und ungefährlicher für Forscher und Umwelt. Kann innerhalb eines Tages Ergebnisse erreicht werden Letztens, kann diese Tecknik für Forschung in Labors gebraucht werden, wo keine Radioaktivität benutzt werden kann." @default.
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- W1983772767 date "1995-01-12" @default.
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- W1983772767 title "Characterization of bovine microsatellites by silver staining" @default.
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- W1983772767 doi "https://doi.org/10.1111/j.1439-0388.1995.tb00580.x" @default.
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