FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983778936> ?p ?o ?g. }

• W1983778936 endingPage "14" @default.
• W1983778936 startingPage "7" @default.
• W1983778936 abstract "Both genome-wide transcriptomic surveys of the mRNA expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus–plant interaction involve the precise estimation of the transcript abundance. Quantitative real-time PCR (qPCR) is the most widely adopted technique for mRNA quantification. In order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. Nevertheless, the stability of internal controls in virus-infected monocots needs to be fully explored. In this work, the suitability of ten housekeeping genes (ACT, EF1α, FBOX, GAPDH, GTPB, PP2A, SAND, TUBβ, UBC18 and UK) for potential use as reference genes in qPCR were investigated in five different monocot plants (Brachypodium, barley, sorghum, wheat and maize) under infection with different viruses including Barley stripe mosaic virus (BSMV), Brome mosaic virus (BMV), Rice black-streaked dwarf virus (RBSDV) and Sugarcane mosaic virus (SCMV). By using three different algorithms, the most appropriate reference genes or their combinations were identified for different experimental sets and their effectiveness for the normalisation of expression studies were further validated by quantitative analysis of a well-studied PR-1 gene. These results facilitate the selection of desirable reference genes for more accurate gene expression studies in virus-infected monocots." @default.
• W1983778936 created "2016-06-24" @default.
• W1983778936 creator A5001640726 @default.
• W1983778936 creator A5003202615 @default.
• W1983778936 creator A5004512291 @default.
• W1983778936 creator A5011901691 @default.
• W1983778936 creator A5033200265 @default.
• W1983778936 creator A5033793255 @default.
• W1983778936 creator A5043821626 @default.
• W1983778936 creator A5044795540 @default.
• W1983778936 creator A5049845476 @default.
• W1983778936 creator A5066064804 @default.
• W1983778936 creator A5075041005 @default.
• W1983778936 creator A5078597351 @default.
• W1983778936 date "2013-10-01" @default.
• W1983778936 modified "2023-09-25" @default.
• W1983778936 title "Selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time PCR" @default.
• W1983778936 cites W1545192385 @default.
• W1983778936 cites W1577577364 @default.
• W1983778936 cites W1980702263 @default.
• W1983778936 cites W1984427878 @default.
• W1983778936 cites W1991946759 @default.
• W1983778936 cites W2010854320 @default.
• W1983778936 cites W2020380193 @default.
• W1983778936 cites W2033406374 @default.
• W1983778936 cites W2044358452 @default.
• W1983778936 cites W2063053724 @default.
• W1983778936 cites W2063094497 @default.
• W1983778936 cites W2063888937 @default.
• W1983778936 cites W2077457607 @default.
• W1983778936 cites W2099824652 @default.
• W1983778936 cites W2103815402 @default.
• W1983778936 cites W2105423509 @default.
• W1983778936 cites W2108244474 @default.
• W1983778936 cites W2110885661 @default.
• W1983778936 cites W2138859454 @default.
• W1983778936 cites W2145141917 @default.
• W1983778936 cites W2154080712 @default.
• W1983778936 cites W2158942814 @default.
• W1983778936 cites W2161525647 @default.
• W1983778936 cites W2163683927 @default.
• W1983778936 cites W2166642834 @default.
• W1983778936 cites W2168420558 @default.
• W1983778936 cites W2169115435 @default.
• W1983778936 cites W2170217346 @default.
• W1983778936 cites W2171154676 @default.
• W1983778936 cites W2171173972 @default.
• W1983778936 doi "https://doi.org/10.1016/j.jbiotec.2013.08.008" @default.
• W1983778936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23954326" @default.
• W1983778936 hasPublicationYear "2013" @default.
• W1983778936 type Work @default.
• W1983778936 sameAs 1983778936 @default.
• W1983778936 citedByCount "31" @default.
• W1983778936 countsByYear W19837789362014 @default.
• W1983778936 countsByYear W19837789362015 @default.
• W1983778936 countsByYear W19837789362016 @default.
• W1983778936 countsByYear W19837789362017 @default.
• W1983778936 countsByYear W19837789362018 @default.
• W1983778936 countsByYear W19837789362019 @default.
• W1983778936 countsByYear W19837789362020 @default.
• W1983778936 countsByYear W19837789362021 @default.
• W1983778936 countsByYear W19837789362022 @default.
• W1983778936 countsByYear W19837789362023 @default.
• W1983778936 crossrefType "journal-article" @default.
• W1983778936 hasAuthorship W1983778936A5001640726 @default.
• W1983778936 hasAuthorship W1983778936A5003202615 @default.
• W1983778936 hasAuthorship W1983778936A5004512291 @default.
• W1983778936 hasAuthorship W1983778936A5011901691 @default.
• W1983778936 hasAuthorship W1983778936A5033200265 @default.
• W1983778936 hasAuthorship W1983778936A5033793255 @default.
• W1983778936 hasAuthorship W1983778936A5043821626 @default.
• W1983778936 hasAuthorship W1983778936A5044795540 @default.
• W1983778936 hasAuthorship W1983778936A5049845476 @default.
• W1983778936 hasAuthorship W1983778936A5066064804 @default.
• W1983778936 hasAuthorship W1983778936A5075041005 @default.
• W1983778936 hasAuthorship W1983778936A5078597351 @default.
• W1983778936 hasConcept C104317684 @default.
• W1983778936 hasConcept C141231307 @default.
• W1983778936 hasConcept C150194340 @default.
• W1983778936 hasConcept C2522874641 @default.
• W1983778936 hasConcept C2777911459 @default.
• W1983778936 hasConcept C2779148672 @default.
• W1983778936 hasConcept C2779535198 @default.
• W1983778936 hasConcept C33922130 @default.
• W1983778936 hasConcept C41258723 @default.
• W1983778936 hasConcept C54355233 @default.
• W1983778936 hasConcept C67705224 @default.
• W1983778936 hasConcept C70721500 @default.
• W1983778936 hasConcept C86803240 @default.
• W1983778936 hasConceptScore W1983778936C104317684 @default.
• W1983778936 hasConceptScore W1983778936C141231307 @default.
• W1983778936 hasConceptScore W1983778936C150194340 @default.
• W1983778936 hasConceptScore W1983778936C2522874641 @default.
• W1983778936 hasConceptScore W1983778936C2777911459 @default.
• W1983778936 hasConceptScore W1983778936C2779148672 @default.
• W1983778936 hasConceptScore W1983778936C2779535198 @default.
• W1983778936 hasConceptScore W1983778936C33922130 @default.
• W1983778936 hasConceptScore W1983778936C41258723 @default.

• next