FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983791720> ?p ?o ?g. }

• W1983791720 endingPage "1151" @default.
• W1983791720 startingPage "1140" @default.
• W1983791720 abstract "MicroRNAs (miRNAs) are short, non-coding RNAs that can negatively regulate expression of multiple genes at post-transcriptional levels. Using miRNAs to target multiple genes and pathways is a promising cell-engineering strategy to increase recombinant protein production in mammalian cells. Here, we identified miRs-17, -19b, -20a, and -92a to be differentially expressed between high- and low- monoclonal antibody-producing Chinese hamster ovary (CHO) cell clones using next-generation sequencing and quantitative real-time PCR. These miRNAs were stably overexpressed individually and in combination in a high-producing clone to assess their effects on CHO cell growth, recombinant protein productivity and product quality. Stably transfected pools demonstrated 24–34% increases in specific productivity (qP) and 21–31% increases in titer relative to the parental clone, without significant alterations in proliferation rates. The highest protein-producing clones isolated from these pools exhibited 130–140% increases in qP and titer compared to the parental clone, without major changes in product aggregation and N-glycosylation profile. From our clonal data, correlations between enhanced qP/titer and increased levels of miRs-17, -19b, and -92a were observed. Our results demonstrate the potential of miRs-17, -19b, and -92a as cell-engineering targets to increase recombinant protein production in mammalian cells." @default.
• W1983791720 created "2016-06-24" @default.
• W1983791720 creator A5002180053 @default.
• W1983791720 creator A5011206845 @default.
• W1983791720 creator A5016267166 @default.
• W1983791720 creator A5026428841 @default.
• W1983791720 creator A5027905163 @default.
• W1983791720 creator A5059576806 @default.
• W1983791720 creator A5061550899 @default.
• W1983791720 date "2014-06-23" @default.
• W1983791720 modified "2023-10-03" @default.
• W1983791720 title "Overexpression of microRNAs enhances recombinant protein production in Chinese hamster ovary cells" @default.
• W1983791720 cites W1969003435 @default.
• W1983791720 cites W1970109552 @default.
• W1983791720 cites W1971464471 @default.
• W1983791720 cites W1974887619 @default.
• W1983791720 cites W1978403430 @default.
• W1983791720 cites W1980167726 @default.
• W1983791720 cites W1983723158 @default.
• W1983791720 cites W1989910375 @default.
• W1983791720 cites W1998653677 @default.
• W1983791720 cites W1999464347 @default.
• W1983791720 cites W2014053766 @default.
• W1983791720 cites W2019148834 @default.
• W1983791720 cites W2023121881 @default.
• W1983791720 cites W2026581189 @default.
• W1983791720 cites W2030501461 @default.
• W1983791720 cites W2034231104 @default.
• W1983791720 cites W2040824502 @default.
• W1983791720 cites W2043436209 @default.
• W1983791720 cites W2044925931 @default.
• W1983791720 cites W2052830113 @default.
• W1983791720 cites W2054414838 @default.
• W1983791720 cites W2055456683 @default.
• W1983791720 cites W2058386777 @default.
• W1983791720 cites W2062290101 @default.
• W1983791720 cites W2065960941 @default.
• W1983791720 cites W2066639891 @default.
• W1983791720 cites W2068160568 @default.
• W1983791720 cites W2068641208 @default.
• W1983791720 cites W2070302019 @default.
• W1983791720 cites W2071702521 @default.
• W1983791720 cites W2071919990 @default.
• W1983791720 cites W2072835990 @default.
• W1983791720 cites W2072886988 @default.
• W1983791720 cites W2074589683 @default.
• W1983791720 cites W2083158631 @default.
• W1983791720 cites W2099993030 @default.
• W1983791720 cites W2107277218 @default.
• W1983791720 cites W2109473940 @default.
• W1983791720 cites W2116365126 @default.
• W1983791720 cites W2121074166 @default.
• W1983791720 cites W2124567772 @default.
• W1983791720 cites W2127071864 @default.
• W1983791720 cites W2128560728 @default.
• W1983791720 cites W2129994868 @default.
• W1983791720 cites W2133968430 @default.
• W1983791720 cites W2137354517 @default.
• W1983791720 cites W2140376253 @default.
• W1983791720 cites W2143090746 @default.
• W1983791720 cites W2146138309 @default.
• W1983791720 cites W2149045044 @default.
• W1983791720 cites W2150203365 @default.
• W1983791720 cites W2163294226 @default.
• W1983791720 cites W2165682628 @default.
• W1983791720 doi "https://doi.org/10.1002/biot.201400050" @default.
• W1983791720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24819042" @default.
• W1983791720 hasPublicationYear "2014" @default.
• W1983791720 type Work @default.
• W1983791720 sameAs 1983791720 @default.
• W1983791720 citedByCount "42" @default.
• W1983791720 countsByYear W19837917202014 @default.
• W1983791720 countsByYear W19837917202015 @default.
• W1983791720 countsByYear W19837917202016 @default.
• W1983791720 countsByYear W19837917202017 @default.
• W1983791720 countsByYear W19837917202018 @default.
• W1983791720 countsByYear W19837917202019 @default.
• W1983791720 countsByYear W19837917202020 @default.
• W1983791720 countsByYear W19837917202021 @default.
• W1983791720 countsByYear W19837917202022 @default.
• W1983791720 countsByYear W19837917202023 @default.
• W1983791720 crossrefType "journal-article" @default.
• W1983791720 hasAuthorship W1983791720A5002180053 @default.
• W1983791720 hasAuthorship W1983791720A5011206845 @default.
• W1983791720 hasAuthorship W1983791720A5016267166 @default.
• W1983791720 hasAuthorship W1983791720A5026428841 @default.
• W1983791720 hasAuthorship W1983791720A5027905163 @default.
• W1983791720 hasAuthorship W1983791720A5059576806 @default.
• W1983791720 hasAuthorship W1983791720A5061550899 @default.
• W1983791720 hasConcept C104317684 @default.
• W1983791720 hasConcept C145059251 @default.
• W1983791720 hasConcept C153911025 @default.
• W1983791720 hasConcept C159654299 @default.
• W1983791720 hasConcept C175656101 @default.
• W1983791720 hasConcept C32611913 @default.
• W1983791720 hasConcept C40767141 @default.
• W1983791720 hasConcept C54009773 @default.
• W1983791720 hasConcept C54355233 @default.

• next