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- W1983802912 abstract "Abstract Tyrosinase is immobilized on controlled-pore glass beads and packed into a stainless-steel column (5 cm × 4 mm i.d.). Serum is deproteinized with tungstate and sulphuric acid. The carrier stream is 0.3 M phosphate buffer (pH 7.2), and is mixed with 5 M potassium hydroxide after the enzyme reactor. The fluorescent dihydroxyindole formed is detected at 490 nm (excitation at 375 nm). The calibration graph is linear for 1 × 10 −7 −1 × 10 −4 M tyrosine; the detection limit is 2 × 10 −8 M." @default.
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- W1983802912 date "1989-01-01" @default.
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- W1983802912 title "Flow-injection determination of l-tyrosine in serum with an immobilized tyrosinase reactor and fluorescence detection" @default.
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- W1983802912 doi "https://doi.org/10.1016/s0003-2670(00)83453-4" @default.
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