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• W1983804962 abstract "DNA sequences (IES elements) eliminated from the developing macronucleus in the ciliate Tetrahymena thermophila are released as linear fragments, which have now been detected and isolated. A PCR-mediated examination of fragment end structures reveals three types of strand scission events, reflecting three steps in the deletion process. New evidence is provided for two steps proposed previously: an initiating double-stranded cleavage, and strand transfer to create a branched deletion intermediate. The fragment ends provide evidence for a previously uncharacterized third step: the branched DNA strand is cleaved at one of several defined sites located within 15-16 nucleotides of the IES boundary, liberating the deleted DNA in a linear form." @default.
• W1983804962 created "2016-06-24" @default.
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• W1983804962 date "2001-06-15" @default.
• W1983804962 modified "2023-10-18" @default.
• W1983804962 title "Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila" @default.
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• W1983804962 doi "https://doi.org/10.1093/emboj/20.12.3251" @default.
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