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- W1983816767 abstract "Proteinase A from Aspergillus niger var. macrosporus is a non-pepsintype acid proteinase, whose catalytic site and enzymatic mechanism remain to be clarified. As the first step toward elucidating its three-dimensional structure by X-ray crystallography, proteinase A was crystallized in ammonium sulfate solutions by the hanging-drop vapor diffusion method. In the absence of an organic solvent, only the aggregates of long, thin needles were formed. On the other hand, thicker rodlike single crystals were grown at around pH 1.75 when dimethylsulfoxide (DMSO) was added to a concentration of around 5%. The single crystals of proteinase A thus obtained were suitable for X-ray diffraction measurements with a precession camera." @default.
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- W1983816767 date "1991-01-01" @default.
- W1983816767 modified "2023-10-01" @default.
- W1983816767 title "Effect of Dimethylsulfoxide on the Crystallization of Aspergillus niger Proteinase A." @default.
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- W1983816767 doi "https://doi.org/10.2183/pjab.67.209" @default.
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