Matches in SemOpenAlex for { <https://semopenalex.org/work/W1983858092> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1983858092 endingPage "535" @default.
- W1983858092 startingPage "533" @default.
- W1983858092 abstract "Hepatocyte growth factor is a recently cloned potent mitogen to hepatocytes, but its extrahe-patic roles are not completely defined. It causes proliferation of endothelial and epithelial cells implicating potential action in the glomerulus. We aimed to determine whether cultured human mesangial cells secrete hepatocyte growth factor and the effect of high glucose conditions. Mesangial cells were isolated from the normal cortex of a child’s kidney. After differential glome-rular sieving and trypsin digestion of glomeruli, mesangial cells were cultured in 20 % fetal calf serum/RPMI. Glucose concentration in the medium was adjusted to 5 mmol/1,11 mmol/1, 25 mmol/1 or 5 mmol/1/20 mmol/1 mannitol to correct for osmolality. After 0, 24, 48, 72 h incubation, hepatocyte growth factor was measured in the supernatant by enzyme immuno assay using recom-binant hepatocyte growth factor and monoclonal antibodies to human hepatocyte growth factor. Hepatocyte growth factor was secreted by cultured mesangial cells. High glucose and hyperosmolar conditions caused a 100-200 % increase in hepatocyte growth factor secretion at 48-72 h (p = 0.001). Hepatocyte growth factor secretion at 48 h in 5 mmol/1 glucose was 16.46 ± 1.09 ng/ml (mean ± SEM), 11 mmol/1 glucose: 32.98 ± 4.54, 25 mmol/1 glucose: 33.32 ±7.89, 5 mmol/1 glucose/20 mmol/1 mannitol: 34.05 ±3.64; at 72 h in 5 mmol/1 glucose: 23.92 ± 2.85 ng/ml, 11 mmol/1 glucose: 28.26 ±2.03, 25 mmo/l glucose: 62.04 ±12.2, 5 mmol/1 glucose/20 mmol/1 mannitol: 45.76 ± 6.25. Trypan blue exclusion demonstrated membrane integrity. These findings demonstrate for the first time that cultured human mesangial cells secrete hepatocyte growth factor and there is stimulation by high glucose and hyperosmolar conditions. Hepatocyte growth factor may have a renotropic role in the pathogenesis of diabetic nephropathy." @default.
- W1983858092 created "2016-06-24" @default.
- W1983858092 creator A5001274627 @default.
- W1983858092 creator A5051169622 @default.
- W1983858092 creator A5063153318 @default.
- W1983858092 creator A5066105917 @default.
- W1983858092 creator A5080102542 @default.
- W1983858092 date "1994-05-01" @default.
- W1983858092 modified "2023-10-03" @default.
- W1983858092 title "High glucose and hyperosmolality stimulate hepatocyte growth factor secretion from cultured human mesangial cells" @default.
- W1983858092 cites W1546402627 @default.
- W1983858092 cites W1977171839 @default.
- W1983858092 cites W2005782838 @default.
- W1983858092 cites W2028804867 @default.
- W1983858092 cites W2035174231 @default.
- W1983858092 cites W2068635604 @default.
- W1983858092 cites W2092661802 @default.
- W1983858092 cites W2155493449 @default.
- W1983858092 cites W225146660 @default.
- W1983858092 doi "https://doi.org/10.1007/s001250050143" @default.
- W1983858092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8056193" @default.
- W1983858092 hasPublicationYear "1994" @default.
- W1983858092 type Work @default.
- W1983858092 sameAs 1983858092 @default.
- W1983858092 citedByCount "10" @default.
- W1983858092 crossrefType "journal-article" @default.
- W1983858092 hasAuthorship W1983858092A5001274627 @default.
- W1983858092 hasAuthorship W1983858092A5051169622 @default.
- W1983858092 hasAuthorship W1983858092A5063153318 @default.
- W1983858092 hasAuthorship W1983858092A5066105917 @default.
- W1983858092 hasAuthorship W1983858092A5080102542 @default.
- W1983858092 hasBestOaLocation W19838580921 @default.
- W1983858092 hasConcept C126322002 @default.
- W1983858092 hasConcept C134018914 @default.
- W1983858092 hasConcept C165220095 @default.
- W1983858092 hasConcept C170493617 @default.
- W1983858092 hasConcept C185592680 @default.
- W1983858092 hasConcept C202751555 @default.
- W1983858092 hasConcept C2775960820 @default.
- W1983858092 hasConcept C2776200302 @default.
- W1983858092 hasConcept C2776996007 @default.
- W1983858092 hasConcept C2778445172 @default.
- W1983858092 hasConcept C2779131090 @default.
- W1983858092 hasConcept C2779306644 @default.
- W1983858092 hasConcept C2780091579 @default.
- W1983858092 hasConcept C2780963292 @default.
- W1983858092 hasConcept C55493867 @default.
- W1983858092 hasConcept C71924100 @default.
- W1983858092 hasConcept C86803240 @default.
- W1983858092 hasConceptScore W1983858092C126322002 @default.
- W1983858092 hasConceptScore W1983858092C134018914 @default.
- W1983858092 hasConceptScore W1983858092C165220095 @default.
- W1983858092 hasConceptScore W1983858092C170493617 @default.
- W1983858092 hasConceptScore W1983858092C185592680 @default.
- W1983858092 hasConceptScore W1983858092C202751555 @default.
- W1983858092 hasConceptScore W1983858092C2775960820 @default.
- W1983858092 hasConceptScore W1983858092C2776200302 @default.
- W1983858092 hasConceptScore W1983858092C2776996007 @default.
- W1983858092 hasConceptScore W1983858092C2778445172 @default.
- W1983858092 hasConceptScore W1983858092C2779131090 @default.
- W1983858092 hasConceptScore W1983858092C2779306644 @default.
- W1983858092 hasConceptScore W1983858092C2780091579 @default.
- W1983858092 hasConceptScore W1983858092C2780963292 @default.
- W1983858092 hasConceptScore W1983858092C55493867 @default.
- W1983858092 hasConceptScore W1983858092C71924100 @default.
- W1983858092 hasConceptScore W1983858092C86803240 @default.
- W1983858092 hasIssue "5" @default.
- W1983858092 hasLocation W19838580921 @default.
- W1983858092 hasLocation W19838580922 @default.
- W1983858092 hasOpenAccess W1983858092 @default.
- W1983858092 hasPrimaryLocation W19838580921 @default.
- W1983858092 hasRelatedWork W1983858092 @default.
- W1983858092 hasRelatedWork W2042033030 @default.
- W1983858092 hasRelatedWork W2044988659 @default.
- W1983858092 hasRelatedWork W2082456910 @default.
- W1983858092 hasRelatedWork W2094423915 @default.
- W1983858092 hasRelatedWork W2098742882 @default.
- W1983858092 hasRelatedWork W2127085070 @default.
- W1983858092 hasRelatedWork W2151391157 @default.
- W1983858092 hasRelatedWork W2400445066 @default.
- W1983858092 hasRelatedWork W2466450716 @default.
- W1983858092 hasVolume "37" @default.
- W1983858092 isParatext "false" @default.
- W1983858092 isRetracted "false" @default.
- W1983858092 magId "1983858092" @default.
- W1983858092 workType "article" @default.