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• W1983872087 endingPage "2528" @default.
• W1983872087 startingPage "2519" @default.
• W1983872087 abstract "Listeria monocytogenes (L. monocytogenes) is a facultative, Gram-positive, food-borne bacterium, which causes serious infections. Although it is known that lipids play important roles in the survival of Listeria, the detailed structures of these lipids have not been established. In this contribution, we described linear ion-trap multiple-stage mass spectrometric approaches with high-resolution mass spectrometry toward complete structural analysis including the identities of the fatty acid substituents and their position on the glycerol backbone of the polar lipids, mainly phosphatidylglycerol, cardiolipin (CL), and lysyl-CL from L. monocytogenes. The location of the methyl side group along the fatty acid chain in each lipid family was characterized by a charge-switch strategy. This is achieved by first alkaline hydrolysis to release the fatty acid substituents, followed by tandem mass spectrometry on their N-(4-aminomethylphenyl) pyridinium (AMPP) derivatives as the M+ ions. Several findings in this study are unique: (1) we confirm the presence of a plasmalogen PG family that has not been previous reported; (2) an ion arising from a rare internal loss of lysylglycerol residue was observed in the MS(2) spectrum of lysyl-CL, permitting its distinction from other CL subfamilies." @default.
• W1983872087 created "2016-06-24" @default.
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• W1983872087 date "2015-02-06" @default.
• W1983872087 modified "2023-10-16" @default.
• W1983872087 title "Characterization of polar lipids of Listeria monocytogenes by HCD and low-energy CAD linear ion-trap mass spectrometry with electrospray ionization" @default.
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• W1983872087 doi "https://doi.org/10.1007/s00216-015-8480-1" @default.
• W1983872087 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4368491" @default.
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