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• W1983917145 abstract "No AccessJournal of UrologyInvestigative Urology1 Dec 2011Spontaneous Ca2+ Signaling of Interstitial Cells in the Guinea Pig Prostate Michelle Lam, Yusuke Shigemasa, Betty Exintaris, Richard J. Lang, and Hikaru Hashitani Michelle LamMichelle Lam Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia More articles by this author , Yusuke ShigemasaYusuke Shigemasa Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan More articles by this author , Betty ExintarisBetty Exintaris Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia More articles by this author , Richard J. LangRichard J. Lang Department of Physiology, Monash University, Clayton, Victoria, Australia More articles by this author , and Hikaru HashitaniHikaru Hashitani Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.07.082AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We investigated whether prostate interstitial cells generate spontaneous Ca2+ oscillation, a proposed mechanism underlying pacemaker potentials to drive spontaneous activity in stromal smooth muscle cells. Materials and Methods: Intracellular free Ca2+ in portions of guinea pig prostate and freshly isolated, single prostate interstitial cells were visualized using fluo-4 Ca2+ fluorescence. Spontaneous electrical activity was recorded in situ with intracellular microelectrodes. Results: In whole tissue preparations spontaneous Ca2+ flashes firing synchronously across all smooth muscle cells within the field of view resulted in muscle wall contractions. Nonpropagating Ca2+ waves were also recorded in individual smooth muscle cells. Nifedipine (Sigma®) (1 μM) largely decreased or abolished these Ca2+ flashes and suppressed slow wave discharge upon blockade of their superimposed action potentials. Isolated prostate interstitial cells were readily distinguished from smooth muscle cells by their spiky processes and lack of contraction during intracellular Ca2+ increases. Prostate interstitial cells generated spontaneous Ca2+ transients in the form of whole cell flashes, intracellular Ca2+ waves or localized Ca2+ sparks. All 3 Ca2+ signals were abolished by nicardipine (1 μM), cyclopiazonic acid (10 μM), caffeine (Sigma) (10 mM) or extracellular Ca2+ removal. Conclusions: Prostate interstitial cells generate spontaneous Ca2+ transients that occur at a frequency comparable to Ca2+ flashes in situ or slow waves relying on functional internal Ca2+ stores. However, unlike other interstitial cells in the urinary tract, Ca2+ influx through L-type Ca2+ channels is fundamental to Ca2+ transient firings in prostate interstitial cells. Thus, it is not possible to conclude that prostate interstitial cells are responsible for pacemaker potential generation. References 1 : Interstitial cells of Cajal as pacemakers in the gastrointestinal tract. Annu Rev Physiol2006; 68: 307. Google Scholar 2 : Mechanisms of disease: specialized interstitial cells of the urinary tract—an assessment of current knowledge. Nat Clin Pract Urol2005; 2: 546. Google Scholar 3 : Properties of spontaneous depolarisations in circular smooth muscle cells of rabbit urethra. Br J Pharmacol1996; 118: 1627. Google Scholar 4 : Specialised pacemaking cells in the rabbit urethra. 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Google Scholar 11 : Role of interstitial cells and gap junctions in the transmission of spontaneous Ca2+ signals in detrusor smooth muscles of the guinea-pig urinary bladder. J Physiol2004; 559: 567. Google Scholar 12 : Spontaneous electrical and Ca2+ signals in typical and atypical smooth muscle cells and interstitial cell of Cajal-like cells of mouse renal pelvis. J Physiol2007; 583: 1049. Google Scholar 13 : Spontaneous slow wave and contractile activity of the guinea pig prostate. J Urol2002; 168: 315. Link, Google Scholar 14 : Characterization of spontaneous depolarizations in smooth muscle cells of the Guinea pig prostate. J Urol2006; 175: 370. Link, Google Scholar 15 : α1-adrenoceptor modulation of spontaneous electrical waveforms in the guinea-pig prostate. Eur J Pharmacol2009; 608: 62. Google Scholar 16 : Characterization of the ion channel currents in single myocytes of the guinea pig prostate. J Urol2004; 172: 1179. Link, Google Scholar 17 : Role of perinuclear mitochondria in the spatiotemporal dynamics of spontaneous Ca2+ waves in interstitial cells of Cajal-like cells of the rabbit urethra. Br J Pharmacol2010; 161: 680. Google Scholar 18 : Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea-pig bladder. Br J Pharmacol2004; 141: 183. Google Scholar 19 : Propagation of pacemaker activity in the guinea-pig antrum. J Physiol2004; 556: 585. Google Scholar 20 : Inositol trisphosphate-dependent Ca stores and mitochondria modulate slow wave activity arising from the smooth muscle cells of the guinea pig prostate gland. Br J Pharmacol2009; 156: 1098. Google Scholar 21 : Role of mitochondria in modulation of spontaneous Ca2+ waves in freshly dispersed interstitial cells of Cajal from the rabbit urethra. J Physiol2008; 586: 4631. Google Scholar 22 : Role of IP3 in modulation of spontaneous activity in pacemaker cells of rabbit urethra. Am J Physiol Cell Physiol2001; 280: C1349. Google Scholar © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byShigemasa Y, Lam M, Mitsui R and Hashitani H (2014) Voltage Dependence of Slow Wave Frequency in the Guinea Pig ProstateJournal of Urology, VOL. 192, NO. 4, (1286-1292), Online publication date: 1-Oct-2014.Lam M, Kerr K and Exintaris B (2012) Involvement of Rho-Kinase Signaling Pathways in Nerve Evoked and Spontaneous Contractions of the Guinea Pig ProstateJournal of Urology, VOL. 189, NO. 3, (1147-1154), Online publication date: 1-Mar-2013. Volume 186Issue 6December 2011Page: 2478-2486 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.Keywordsprostatecalcium channelssmooth muscleaction potentialsL-typemyocytesmuscle contractionMetricsAuthor Information Michelle Lam Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia More articles by this author Yusuke Shigemasa Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan More articles by this author Betty Exintaris Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia More articles by this author Richard J. Lang Department of Physiology, Monash University, Clayton, Victoria, Australia More articles by this author Hikaru Hashitani Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W1983917145 title "Spontaneous Ca ²⁺ Signaling of Interstitial Cells in the Guinea Pig Prostate" @default.
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