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- W1983968064 abstract "The reactivity of the five main histones in calf thymus chromatin towards acetic anhydride has been determined using the technique of competitive labelling (Kaplan et al., 1971; Kaplan, 1972). Under trace labelling conditions all the histones incorporate radioactive label from tritiated acetic anhydride, however, only histone IIb2 incorporates the label at a rate which is consistent with a substantial portion of its amino groups being fully exposed. Identification of the reactive functional groups of histone IIb2, revealed that the reactivity could be accounted for entirely by the reactivity of its N-terminal proline. It is concluded that the vast majority if not all the ϵ-amino groups of histones in calf thymus chromatin have abnormally high pKa values and thus appear to be buried, while the N-terminus of histone IIb2 is exposed. This conclusion supports the hypothesis that the ϵ-amino groups of histones form salt linkages with the phosphates of DNA." @default.
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- W1983968064 date "1974-02-01" @default.
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- W1983968064 title "Reactive properties of the amino groups of histones in calf thymus chromatin" @default.
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- W1983968064 doi "https://doi.org/10.1016/0022-2836(74)90247-2" @default.
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