FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984172682> ?p ?o ?g. }

• W1984172682 endingPage "26208" @default.
• W1984172682 startingPage "26202" @default.
• W1984172682 abstract "Dithiocarbamates are metal-chelating compounds that can exert either pro-oxidant or antioxidant effects in different situations. They have recently been found to potently inhibit apoptotic cell death, an activity attributed to their antioxidant action. However, when thymocytes were exposed to pyrrolidine dithiocarbamate, an oxidation of the glutathione pool occurred within 90 min. Longer incubation resulted in cell shrinkage, chromatin fragmentation, glutathione depletion, and eventual cell lysis, which is typical of apoptosis in these cells. These changes were inhibited by inclusion of non-permeable metal chelators in the incubation medium, suggesting that pyrrolidine dithiocarbamate exerts its toxic effect by transporting a redox-active metal into the cell. This was directly confirmed when sustained 8-fold elevations of intracellular copper were detected after addition of pyrrolidine dithiocarbamate. In agreement with this, supplementation of the incubation medium with submicromolar concentrations of copper significantly potentiated pyrrolidine dithiocarbamate toxicity. We conclude that pyrrolidine dithiocarbamate exerts a powerful pro-oxidant effect on thymocytes due to its ability to transport external redox-active copper into cells. The resulting increase in glutathione disulfide may also explain the temporary anti-apoptotic activity of this compound described in other systems. Dithiocarbamates are metal-chelating compounds that can exert either pro-oxidant or antioxidant effects in different situations. They have recently been found to potently inhibit apoptotic cell death, an activity attributed to their antioxidant action. However, when thymocytes were exposed to pyrrolidine dithiocarbamate, an oxidation of the glutathione pool occurred within 90 min. Longer incubation resulted in cell shrinkage, chromatin fragmentation, glutathione depletion, and eventual cell lysis, which is typical of apoptosis in these cells. These changes were inhibited by inclusion of non-permeable metal chelators in the incubation medium, suggesting that pyrrolidine dithiocarbamate exerts its toxic effect by transporting a redox-active metal into the cell. This was directly confirmed when sustained 8-fold elevations of intracellular copper were detected after addition of pyrrolidine dithiocarbamate. In agreement with this, supplementation of the incubation medium with submicromolar concentrations of copper significantly potentiated pyrrolidine dithiocarbamate toxicity. We conclude that pyrrolidine dithiocarbamate exerts a powerful pro-oxidant effect on thymocytes due to its ability to transport external redox-active copper into cells. The resulting increase in glutathione disulfide may also explain the temporary anti-apoptotic activity of this compound described in other systems." @default.
• W1984172682 created "2016-06-24" @default.
• W1984172682 creator A5027036360 @default.
• W1984172682 creator A5052727064 @default.
• W1984172682 creator A5060371731 @default.
• W1984172682 creator A5075563011 @default.
• W1984172682 creator A5084588481 @default.
• W1984172682 date "1995-11-01" @default.
• W1984172682 modified "2023-09-30" @default.
• W1984172682 title "Dithiocarbamates Induce Apoptosis in Thymocytes by Raising the Intracellular Level of Redox-active Copper" @default.
• W1984172682 cites W1480914201 @default.
• W1984172682 cites W1484365178 @default.
• W1984172682 cites W1536792772 @default.
• W1984172682 cites W1550882311 @default.
• W1984172682 cites W1571251056 @default.
• W1984172682 cites W1577403013 @default.
• W1984172682 cites W1579604097 @default.
• W1984172682 cites W1598210890 @default.
• W1984172682 cites W1734082729 @default.
• W1984172682 cites W1856122700 @default.
• W1984172682 cites W1949043027 @default.
• W1984172682 cites W1970749702 @default.
• W1984172682 cites W1981748417 @default.
• W1984172682 cites W1988921994 @default.
• W1984172682 cites W1988951544 @default.
• W1984172682 cites W2004878906 @default.
• W1984172682 cites W2005416997 @default.
• W1984172682 cites W2014078849 @default.
• W1984172682 cites W2024604097 @default.
• W1984172682 cites W2029184130 @default.
• W1984172682 cites W2031254695 @default.
• W1984172682 cites W2050576725 @default.
• W1984172682 cites W2055810975 @default.
• W1984172682 cites W2057391801 @default.
• W1984172682 cites W2061368292 @default.
• W1984172682 cites W2064280448 @default.
• W1984172682 cites W2068001141 @default.
• W1984172682 cites W2069068041 @default.
• W1984172682 cites W2074932060 @default.
• W1984172682 cites W2084153771 @default.
• W1984172682 cites W2091555270 @default.
• W1984172682 cites W2093263978 @default.
• W1984172682 cites W2112989132 @default.
• W1984172682 cites W2135073428 @default.
• W1984172682 cites W2138098641 @default.
• W1984172682 cites W2144377798 @default.
• W1984172682 cites W2144605551 @default.
• W1984172682 cites W2152719751 @default.
• W1984172682 cites W2159567970 @default.
• W1984172682 cites W2215510318 @default.
• W1984172682 cites W344575546 @default.
• W1984172682 doi "https://doi.org/10.1074/jbc.270.44.26202" @default.
• W1984172682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7592825" @default.
• W1984172682 hasPublicationYear "1995" @default.
• W1984172682 type Work @default.
• W1984172682 sameAs 1984172682 @default.
• W1984172682 citedByCount "265" @default.
• W1984172682 countsByYear W19841726822012 @default.
• W1984172682 countsByYear W19841726822013 @default.
• W1984172682 countsByYear W19841726822014 @default.
• W1984172682 countsByYear W19841726822015 @default.
• W1984172682 countsByYear W19841726822016 @default.
• W1984172682 countsByYear W19841726822017 @default.
• W1984172682 countsByYear W19841726822018 @default.
• W1984172682 countsByYear W19841726822019 @default.
• W1984172682 countsByYear W19841726822020 @default.
• W1984172682 countsByYear W19841726822021 @default.
• W1984172682 countsByYear W19841726822022 @default.
• W1984172682 countsByYear W19841726822023 @default.
• W1984172682 crossrefType "journal-article" @default.
• W1984172682 hasAuthorship W1984172682A5027036360 @default.
• W1984172682 hasAuthorship W1984172682A5052727064 @default.
• W1984172682 hasAuthorship W1984172682A5060371731 @default.
• W1984172682 hasAuthorship W1984172682A5075563011 @default.
• W1984172682 hasAuthorship W1984172682A5084588481 @default.
• W1984172682 hasBestOaLocation W19841726821 @default.
• W1984172682 hasConcept C178790620 @default.
• W1984172682 hasConcept C181199279 @default.
• W1984172682 hasConcept C185592680 @default.
• W1984172682 hasConcept C190283241 @default.
• W1984172682 hasConcept C2777352226 @default.
• W1984172682 hasConcept C2777545050 @default.
• W1984172682 hasConcept C2777730290 @default.
• W1984172682 hasConcept C2778004101 @default.
• W1984172682 hasConcept C2780829032 @default.
• W1984172682 hasConcept C2780983412 @default.
• W1984172682 hasConcept C31573885 @default.
• W1984172682 hasConcept C538909803 @default.
• W1984172682 hasConcept C55493867 @default.
• W1984172682 hasConcept C79879829 @default.
• W1984172682 hasConceptScore W1984172682C178790620 @default.
• W1984172682 hasConceptScore W1984172682C181199279 @default.
• W1984172682 hasConceptScore W1984172682C185592680 @default.
• W1984172682 hasConceptScore W1984172682C190283241 @default.
• W1984172682 hasConceptScore W1984172682C2777352226 @default.
• W1984172682 hasConceptScore W1984172682C2777545050 @default.
• W1984172682 hasConceptScore W1984172682C2777730290 @default.
• W1984172682 hasConceptScore W1984172682C2778004101 @default.

• next