Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984177316> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1984177316 abstract "DNA fragments generated by a variety of restriction enzymes can easily be cloned in the small (3.2-kb) positive-selection vector pUH84, which contains the modified lysis gene of bacteriophage phi X174 under transcriptional control of the lac promoter. Plasmid pUH84 does not yield transformants after introduction into Escherichia coli unless the lysis gene is inactivated by insertion of foreign DNA into one of the unique PstI, SalI, AccI, HincII, BamHI, or EcoRI sites. This highly efficient positive selection of recombinants requires neither the use of a distinct host strain nor a special induction of the lysis function. Transcription of fragments cloned into pUH84 may be effectively regulated by the lac promoter provided the host cells are cotransformed with the newly constructed plasmid pUH7 which carries the IQ allele of the lac repressor gene." @default.
- W1984177316 created "2016-06-24" @default.
- W1984177316 creator A5010851139 @default.
- W1984177316 creator A5039318497 @default.
- W1984177316 date "1986-01-01" @default.
- W1984177316 modified "2023-09-25" @default.
- W1984177316 title "Use of the lysis gene of bacteriophage φX174 for the construction of a positive selection vector" @default.
- W1984177316 cites W1571716654 @default.
- W1984177316 cites W1963513493 @default.
- W1984177316 cites W1963940836 @default.
- W1984177316 cites W1969094530 @default.
- W1984177316 cites W1994469830 @default.
- W1984177316 cites W1994476689 @default.
- W1984177316 cites W1998247911 @default.
- W1984177316 cites W2022064097 @default.
- W1984177316 cites W2026390749 @default.
- W1984177316 cites W2071659208 @default.
- W1984177316 cites W2094975937 @default.
- W1984177316 cites W2117476471 @default.
- W1984177316 cites W2141653906 @default.
- W1984177316 cites W2143301841 @default.
- W1984177316 cites W2189084452 @default.
- W1984177316 cites W2327587570 @default.
- W1984177316 cites W238813722 @default.
- W1984177316 cites W2090920017 @default.
- W1984177316 doi "https://doi.org/10.1016/0378-1119(86)90239-8" @default.
- W1984177316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2942443" @default.
- W1984177316 hasPublicationYear "1986" @default.
- W1984177316 type Work @default.
- W1984177316 sameAs 1984177316 @default.
- W1984177316 citedByCount "29" @default.
- W1984177316 countsByYear W19841773162018 @default.
- W1984177316 countsByYear W19841773162021 @default.
- W1984177316 crossrefType "journal-article" @default.
- W1984177316 hasAuthorship W1984177316A5010851139 @default.
- W1984177316 hasAuthorship W1984177316A5039318497 @default.
- W1984177316 hasConcept C104317684 @default.
- W1984177316 hasConcept C128319531 @default.
- W1984177316 hasConcept C130123407 @default.
- W1984177316 hasConcept C136199206 @default.
- W1984177316 hasConcept C150194340 @default.
- W1984177316 hasConcept C153911025 @default.
- W1984177316 hasConcept C158448853 @default.
- W1984177316 hasConcept C22744801 @default.
- W1984177316 hasConcept C2776441376 @default.
- W1984177316 hasConcept C2780397633 @default.
- W1984177316 hasConcept C42174275 @default.
- W1984177316 hasConcept C54355233 @default.
- W1984177316 hasConcept C547475151 @default.
- W1984177316 hasConcept C552990157 @default.
- W1984177316 hasConcept C86803240 @default.
- W1984177316 hasConceptScore W1984177316C104317684 @default.
- W1984177316 hasConceptScore W1984177316C128319531 @default.
- W1984177316 hasConceptScore W1984177316C130123407 @default.
- W1984177316 hasConceptScore W1984177316C136199206 @default.
- W1984177316 hasConceptScore W1984177316C150194340 @default.
- W1984177316 hasConceptScore W1984177316C153911025 @default.
- W1984177316 hasConceptScore W1984177316C158448853 @default.
- W1984177316 hasConceptScore W1984177316C22744801 @default.
- W1984177316 hasConceptScore W1984177316C2776441376 @default.
- W1984177316 hasConceptScore W1984177316C2780397633 @default.
- W1984177316 hasConceptScore W1984177316C42174275 @default.
- W1984177316 hasConceptScore W1984177316C54355233 @default.
- W1984177316 hasConceptScore W1984177316C547475151 @default.
- W1984177316 hasConceptScore W1984177316C552990157 @default.
- W1984177316 hasConceptScore W1984177316C86803240 @default.
- W1984177316 hasLocation W19841773161 @default.
- W1984177316 hasLocation W19841773162 @default.
- W1984177316 hasOpenAccess W1984177316 @default.
- W1984177316 hasPrimaryLocation W19841773161 @default.
- W1984177316 hasRelatedWork W1977681177 @default.
- W1984177316 hasRelatedWork W2022172313 @default.
- W1984177316 hasRelatedWork W2034491972 @default.
- W1984177316 hasRelatedWork W2415898635 @default.
- W1984177316 hasRelatedWork W2433966938 @default.
- W1984177316 hasRelatedWork W2468725391 @default.
- W1984177316 hasRelatedWork W4290367698 @default.
- W1984177316 hasRelatedWork W4290369313 @default.
- W1984177316 hasRelatedWork W66908765 @default.
- W1984177316 hasRelatedWork W2612387522 @default.
- W1984177316 isParatext "false" @default.
- W1984177316 isRetracted "false" @default.
- W1984177316 magId "1984177316" @default.
- W1984177316 workType "article" @default.