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- W1984231322 abstract "An osmotic swelling assay utilising carboxyfluorescein self-quenching to measure intravesicular volume changes was adapted to investigate permeability changes induced by the Bacillus thuringiensis Cry1Ac delta-endotoxin in Manduca sexta midgut-brush-border-membrane vesicles (BBMV). This assay provides a more quantitative analysis of Cry-toxin-induced BBMV permeability changes, extending our previously published protocol which employed a light-scattering signal to monitor delta-endotoxin activity [Carroll, J. & Ellar, D. J. (1993) Eur. J. Biochem. 214, 771-778]. The fluorescence signal changes, supported by electron microscopy of the BBMV, demonstrated that Cry1Ac altered the membrane permeability for large non-electrolyte solutes. With this approach Cry1Ac was observed to induce or form pores freely permeant for raffinose (1.14 nm diameter) and using non-electrolytes of increasing size the pores were estimated to have a limiting diameter of approximately 2.4-2.6 nm under alkaline pH conditions." @default.
- W1984231322 created "2016-06-24" @default.
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- W1984231322 date "1997-05-01" @default.
- W1984231322 modified "2023-09-27" @default.
- W1984231322 title "Analysis of the Large Aqueous Pores Produced by a Bacillus Turingiensis Protein Insecticide in Manduca Sexta Midgut-Brush-Border-Membrane Vesicles" @default.
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- W1984231322 doi "https://doi.org/10.1111/j.1432-1033.1997.00797.x" @default.
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