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- W1984279087 abstract "A rapid full-spectrum recording spectrophotometer was used to analyze the nucleotide composition of a yeast cell extract by high-performance liquid chromatography. Throughout the run, which lasted 47 min, a UV spectrum with 1-nm resolution was transmitted to a minicomputer every 2.5 sec. The recorded data were later evaluated by several computer programs. One program (HPMAX) plots the position of the absorption maximum for each time point. Another program (HPSPEC) displays three spectra at any chosen time points and enables their normalization (allowing comparison with standards and analysis of the purity of peaks) and the display of the logarithm of the ratio of the values (used to determine the best wavelength for integration of overlapping peaks). A third program (HPEAK) displays the absorption at any desired wavelength against time, allows cursor positioning to mark the position of a slanting baseline as well as the beginning and end of a peak, and the integration of the peak. The components in overlapping peaks can be separately integrated. The method is generally applicable to all compounds that are detectable by spectrophotometric means." @default.
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- W1984279087 date "1984-01-01" @default.
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- W1984279087 title "Nucleotide composition of cell extracts analyzed by full-spectrum recording in high-performance liquid chromatography" @default.
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- W1984279087 doi "https://doi.org/10.1016/s0021-9673(01)87808-0" @default.
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