FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984291280> ?p ?o ?g. }

• W1984291280 abstract "Pulmonary-delivered gene therapy promises to mitigate vaccine safety issues and reduce the need for needles and skilled personnel to use them. While plasmid DNA (pDNA) offers a rapid route to vaccine production without side effects or reliance on cold chain storage, its delivery to the lung has proved challenging. Conventional methods, including jet and ultrasonic nebulizers, fail to deliver large biomolecules like pDNA intact due to the shear and cavitational stresses present during nebulization. In vitro structural analysis followed by in vivo protein expression studies served in assessing the integrity of the pDNA subjected to surface acoustic wave (SAW) nebulisation. In vivo immunization trials were then carried out in rats using SAW nebulized pDNA (influenza A, human hemagglutinin H1N1) condensate delivered via intratracheal instillation. Finally, in vivo pulmonary vaccinations using pDNA for influenza was nebulized and delivered via a respirator to sheep. The SAW nebulizer was effective at generating pDNA aerosols with sizes optimal for deep lung delivery. Successful gene expression was observed in mouse lung epithelial cells, when SAW-nebulized pDNA was delivered to male Swiss mice via intratracheal instillation. Effective systemic and mucosal antibody responses were found in rats via post-nebulized, condensed fluid instillation. Significantly, we demonstrated the suitability of the SAW nebulizer to administer unprotected pDNA encoding an influenza A virus surface glycoprotein to respirated sheep via aerosolized inhalation. Given the difficulty of inducing functional antibody responses for DNA vaccination in large animals, we report here the first instance of successful aerosolized inhalation delivery of a pDNA vaccine in a large animal model relevant to human lung development, structure, physiology, and disease, using a novel, low-power (<1 W) surface acoustic wave (SAW) hand-held nebulizer to produce droplets of pDNA with a size range suitable for delivery to the lower respiratory airways." @default.
• W1984291280 created "2016-06-24" @default.
• W1984291280 creator A5017582467 @default.
• W1984291280 creator A5024137694 @default.
• W1984291280 creator A5025528085 @default.
• W1984291280 creator A5035953102 @default.
• W1984291280 creator A5048595089 @default.
• W1984291280 creator A5058201833 @default.
• W1984291280 creator A5061604556 @default.
• W1984291280 creator A5080905920 @default.
• W1984291280 creator A5084975321 @default.
• W1984291280 creator A5088496687 @default.
• W1984291280 creator A5088932188 @default.
• W1984291280 creator A5007437989 @default.
• W1984291280 date "2014-05-20" @default.
• W1984291280 modified "2023-10-18" @default.
• W1984291280 title "Effective pulmonary delivery of an aerosolized plasmid DNA vaccine via surface acoustic wave nebulization" @default.
• W1984291280 cites W1967768293 @default.
• W1984291280 cites W1968284072 @default.
• W1984291280 cites W1974288028 @default.
• W1984291280 cites W1974550668 @default.
• W1984291280 cites W1984471128 @default.
• W1984291280 cites W1988068031 @default.
• W1984291280 cites W1993882958 @default.
• W1984291280 cites W1994588824 @default.
• W1984291280 cites W1995997091 @default.
• W1984291280 cites W2001132167 @default.
• W1984291280 cites W2001986771 @default.
• W1984291280 cites W2007146866 @default.
• W1984291280 cites W2008126601 @default.
• W1984291280 cites W2018450825 @default.
• W1984291280 cites W2020419107 @default.
• W1984291280 cites W2024055277 @default.
• W1984291280 cites W2042307666 @default.
• W1984291280 cites W2044524458 @default.
• W1984291280 cites W2051226833 @default.
• W1984291280 cites W2055802294 @default.
• W1984291280 cites W2059459218 @default.
• W1984291280 cites W2060031655 @default.
• W1984291280 cites W2060202224 @default.
• W1984291280 cites W2061077904 @default.
• W1984291280 cites W2070357368 @default.
• W1984291280 cites W20717472 @default.
• W1984291280 cites W2072316358 @default.
• W1984291280 cites W2088074649 @default.
• W1984291280 cites W2091584013 @default.
• W1984291280 cites W2104946399 @default.
• W1984291280 cites W2105114931 @default.
• W1984291280 cites W2111398700 @default.
• W1984291280 cites W2112333290 @default.
• W1984291280 cites W2115264660 @default.
• W1984291280 cites W2120416061 @default.
• W1984291280 cites W2134004194 @default.
• W1984291280 cites W2139117182 @default.
• W1984291280 cites W2143871880 @default.
• W1984291280 cites W2145674936 @default.
• W1984291280 cites W2168675734 @default.
• W1984291280 cites W2169325688 @default.
• W1984291280 cites W2169383781 @default.
• W1984291280 cites W4233909860 @default.
• W1984291280 doi "https://doi.org/10.1186/1465-9921-15-60" @default.
• W1984291280 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4040411" @default.
• W1984291280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24884387" @default.
• W1984291280 hasPublicationYear "2014" @default.
• W1984291280 type Work @default.
• W1984291280 sameAs 1984291280 @default.
• W1984291280 citedByCount "75" @default.
• W1984291280 countsByYear W19842912802014 @default.
• W1984291280 countsByYear W19842912802015 @default.
• W1984291280 countsByYear W19842912802016 @default.
• W1984291280 countsByYear W19842912802017 @default.
• W1984291280 countsByYear W19842912802018 @default.
• W1984291280 countsByYear W19842912802019 @default.
• W1984291280 countsByYear W19842912802020 @default.
• W1984291280 countsByYear W19842912802021 @default.
• W1984291280 countsByYear W19842912802022 @default.
• W1984291280 countsByYear W19842912802023 @default.
• W1984291280 crossrefType "journal-article" @default.
• W1984291280 hasAuthorship W1984291280A5007437989 @default.
• W1984291280 hasAuthorship W1984291280A5017582467 @default.
• W1984291280 hasAuthorship W1984291280A5024137694 @default.
• W1984291280 hasAuthorship W1984291280A5025528085 @default.
• W1984291280 hasAuthorship W1984291280A5035953102 @default.
• W1984291280 hasAuthorship W1984291280A5048595089 @default.
• W1984291280 hasAuthorship W1984291280A5058201833 @default.
• W1984291280 hasAuthorship W1984291280A5061604556 @default.
• W1984291280 hasAuthorship W1984291280A5080905920 @default.
• W1984291280 hasAuthorship W1984291280A5084975321 @default.
• W1984291280 hasAuthorship W1984291280A5088496687 @default.
• W1984291280 hasAuthorship W1984291280A5088932188 @default.
• W1984291280 hasBestOaLocation W19842912801 @default.
• W1984291280 hasConcept C104317684 @default.
• W1984291280 hasConcept C111599444 @default.
• W1984291280 hasConcept C126322002 @default.
• W1984291280 hasConcept C135983454 @default.
• W1984291280 hasConcept C150194340 @default.
• W1984291280 hasConcept C150903083 @default.
• W1984291280 hasConcept C159654299 @default.
• W1984291280 hasConcept C161733203 @default.
• W1984291280 hasConcept C167508917 @default.

• next