FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984333260> ?p ?o ?g. }

• W1984333260 endingPage "568" @default.
• W1984333260 startingPage "559" @default.
• W1984333260 abstract "Abstract We present data that reveal crucial differences between the binding mode of anti‐gastrin17 (G17, pyro EGPWLEEEEEAYGWMDF‐NH 2 ) monoclonal antibodies (mAbs) and their CDR‐derived synthetic binders (SBs) with G17. The mAbs recognize the N‐terminal sequence of G17 ( pyro EGPWL) with nanomolar affinity and high sequence selectivity. Molecular simulations suggest that G17 recognition is based primarily on a multitude of weak antibody–ligand interactions (H‐bonding, van der Waals, etc.) inside a structurally well‐defined cleft‐like binding pocket. Relatively small structural changes (e.g. G‐2 to A for G17) have a drastic impact on affinity, which is characteristic for antibody‐like binding. In contrast, SBs recognize various sequences, including G17‐unrelated targets with affinities of 1:1 complexes estimated in the 0.1–1.0 mM range. In most cases however, the G17/SB complex stoichiometries are not well‐defined, giving rise to multimer aggregate formation with high apparent complex stabilities. Mutational studies on both G17 and SBs reveal the importance of positively charged (K/R) and aromatic residues (W/Y/F) for G17/SB complex formation. We propose that the synthetic binders use combinations of electrostatic, hydrophobic, and/or cation–π interactions in a variety of ways due to their intrinsic flexibility. This may also be the reason for their relatively low target specificity. We speculate that our findings are of general relevance, in showing that high‐affinity mAbs do not necessarily provide the optimal basis for functional mimics design. Copyright © 2010 John Wiley & Sons, Ltd." @default.
• W1984333260 created "2016-06-24" @default.
• W1984333260 creator A5008299857 @default.
• W1984333260 creator A5038069029 @default.
• W1984333260 creator A5061380586 @default.
• W1984333260 creator A5061636613 @default.
• W1984333260 creator A5066078399 @default.
• W1984333260 creator A5077088234 @default.
• W1984333260 creator A5080722826 @default.
• W1984333260 date "2010-10-28" @default.
• W1984333260 modified "2023-09-27" @default.
• W1984333260 title "Binding of CDR-derived peptides is mechanistically different from that of high-affinity parental antibodies" @default.
• W1984333260 cites W105839754 @default.
• W1984333260 cites W1495563488 @default.
• W1984333260 cites W1664648378 @default.
• W1984333260 cites W1969609139 @default.
• W1984333260 cites W1977239232 @default.
• W1984333260 cites W1977572092 @default.
• W1984333260 cites W1981848510 @default.
• W1984333260 cites W1986809692 @default.
• W1984333260 cites W1990704592 @default.
• W1984333260 cites W1992227523 @default.
• W1984333260 cites W1997740773 @default.
• W1984333260 cites W2010178169 @default.
• W1984333260 cites W2013645466 @default.
• W1984333260 cites W2017557874 @default.
• W1984333260 cites W2018494763 @default.
• W1984333260 cites W2019401962 @default.
• W1984333260 cites W2026649090 @default.
• W1984333260 cites W2026971673 @default.
• W1984333260 cites W2042313745 @default.
• W1984333260 cites W2044868850 @default.
• W1984333260 cites W2044875778 @default.
• W1984333260 cites W2048710976 @default.
• W1984333260 cites W2054720672 @default.
• W1984333260 cites W2058000016 @default.
• W1984333260 cites W2066714607 @default.
• W1984333260 cites W2071230199 @default.
• W1984333260 cites W2074166694 @default.
• W1984333260 cites W2080622373 @default.
• W1984333260 cites W2093618292 @default.
• W1984333260 cites W2094320255 @default.
• W1984333260 cites W2137450588 @default.
• W1984333260 cites W2147344136 @default.
• W1984333260 cites W2149979517 @default.
• W1984333260 cites W2155726970 @default.
• W1984333260 cites W2159172675 @default.
• W1984333260 cites W2159537259 @default.
• W1984333260 cites W2161097929 @default.
• W1984333260 cites W2163598955 @default.
• W1984333260 cites W2169743816 @default.
• W1984333260 cites W2170578143 @default.
• W1984333260 doi "https://doi.org/10.1002/jmr.1017" @default.
• W1984333260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21038356" @default.
• W1984333260 hasPublicationYear "2010" @default.
• W1984333260 type Work @default.
• W1984333260 sameAs 1984333260 @default.
• W1984333260 citedByCount "7" @default.
• W1984333260 countsByYear W19843332602013 @default.
• W1984333260 countsByYear W19843332602015 @default.
• W1984333260 countsByYear W19843332602016 @default.
• W1984333260 countsByYear W19843332602017 @default.
• W1984333260 countsByYear W19843332602022 @default.
• W1984333260 crossrefType "journal-article" @default.
• W1984333260 hasAuthorship W1984333260A5008299857 @default.
• W1984333260 hasAuthorship W1984333260A5038069029 @default.
• W1984333260 hasAuthorship W1984333260A5061380586 @default.
• W1984333260 hasAuthorship W1984333260A5061636613 @default.
• W1984333260 hasAuthorship W1984333260A5066078399 @default.
• W1984333260 hasAuthorship W1984333260A5077088234 @default.
• W1984333260 hasAuthorship W1984333260A5080722826 @default.
• W1984333260 hasBestOaLocation W19843332601 @default.
• W1984333260 hasConcept C104317684 @default.
• W1984333260 hasConcept C116569031 @default.
• W1984333260 hasConcept C118792377 @default.
• W1984333260 hasConcept C12554922 @default.
• W1984333260 hasConcept C126061179 @default.
• W1984333260 hasConcept C159654299 @default.
• W1984333260 hasConcept C161790260 @default.
• W1984333260 hasConcept C167625842 @default.
• W1984333260 hasConcept C170493617 @default.
• W1984333260 hasConcept C178790620 @default.
• W1984333260 hasConcept C185592680 @default.
• W1984333260 hasConcept C2778112365 @default.
• W1984333260 hasConcept C2780283098 @default.
• W1984333260 hasConcept C3018795828 @default.
• W1984333260 hasConcept C32909587 @default.
• W1984333260 hasConcept C542903549 @default.
• W1984333260 hasConcept C54355233 @default.
• W1984333260 hasConcept C55493867 @default.
• W1984333260 hasConcept C71240020 @default.
• W1984333260 hasConcept C86803240 @default.
• W1984333260 hasConceptScore W1984333260C104317684 @default.
• W1984333260 hasConceptScore W1984333260C116569031 @default.
• W1984333260 hasConceptScore W1984333260C118792377 @default.
• W1984333260 hasConceptScore W1984333260C12554922 @default.
• W1984333260 hasConceptScore W1984333260C126061179 @default.
• W1984333260 hasConceptScore W1984333260C159654299 @default.

• next