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• W1984335423 abstract "A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as putative interacting proteins using mass spectrometry. Control experiments can be used to limit false-positive results, but in many cases, there are still a surprising number of prey proteins that appear to copurify specifically with the bait. Here, we have identified one source of false-positive interactions in such studies. We have found that a combination of: 1) the variable sequence of the C-terminus of the bait with 2) a C-terminal valine cloning scar present in a commercially available ORF library, can in some cases create a peptide motif that results in the aberrant co-purification of endogenous cellular proteins. Control experiments may not identify false positives resulting from such artificial motifs, as aberrant binding depends on sequences that vary from one bait to another. It is possible that such cryptic protein binding might occur in other systems using affinity tagged proteins; this study highlights the importance of conducting careful follow-up studies where novel protein-protein interactions are suspected." @default.
• W1984335423 created "2016-06-24" @default.
• W1984335423 creator A5034566006 @default.
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• W1984335423 date "2015-02-23" @default.
• W1984335423 modified "2023-10-16" @default.
• W1984335423 title "Proteins interacting with cloning scars: a source of false positive protein-protein interactions" @default.
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• W1984335423 doi "https://doi.org/10.1038/srep08530" @default.
• W1984335423 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4336944" @default.
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• W1984335423 hasPublicationYear "2015" @default.
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