FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984432487> ?p ?o ?g. }

• W1984432487 endingPage "977" @default.
• W1984432487 startingPage "968" @default.
• W1984432487 abstract "In this work, a robust synthetic nanostructure was designed for the effective packaging of DNA and it was shown to be an efficient agent for cell transfection. An amphiphilic block copolymer, poly(acrylamidoethylamine)128-b-polystyrene40 (PAEA128-b-PS40), was synthesized, micellized in water and shell-crosslinked using a diacid-derivatized crosslinker, to give cationic shell-crosslinked nanoparticles (cSCKs) with a mean hydrodynamic diameter of 14 ± 2 nm. A series of discrete complexes of the cSCKs with plasmid DNA (pDNA) was able to be formed over a broad range of polymer amine:pDNA phosphate ratios (N/P ratio), 2:1–20:1. The sizes of the complexes and their ability to fully bind the pDNA were dependent upon the N/P ratio, as characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM) and gel retardation assay. A luciferase activity assay and EGFP expression were used to evaluate intracellular delivery of a splice-correcting phosphorothioate and genetic material, respectively, by the cSCKs, which indicated that an N/P ratio of 6:1 gave the highest transfection. It was shown by both luciferase activity assay (48 h) and EGFP transfection data that high transfection efficiencies were achieved for HeLa cells transfected by cSCK/CCUCUUACCUCAGUUACA and cSCK/pEGFP-N1 plasmid, respectively. The cSCK/pEGFP-N1 plasmid transfection efficiency of 27% far exceeded the performance of Polyfect® (PAMAM dendrimers), which achieved only 12% transfection efficiency, under the same conditions. Cytotoxicities for the cSCKs were evaluated for HeLa and CHO cells." @default.
• W1984432487 created "2016-06-24" @default.
• W1984432487 creator A5030064573 @default.
• W1984432487 creator A5079724569 @default.
• W1984432487 creator A5082819905 @default.
• W1984432487 creator A5083138002 @default.
• W1984432487 creator A5087261496 @default.
• W1984432487 date "2009-02-01" @default.
• W1984432487 modified "2023-10-17" @default.
• W1984432487 title "Cationic shell-crosslinked knedel-like nanoparticles for highly efficient gene and oligonucleotide transfection of mammalian cells" @default.
• W1984432487 cites W1616824889 @default.
• W1984432487 cites W1636022800 @default.
• W1984432487 cites W1822988701 @default.
• W1984432487 cites W1948127231 @default.
• W1984432487 cites W1977860513 @default.
• W1984432487 cites W2001181075 @default.
• W1984432487 cites W2003336829 @default.
• W1984432487 cites W2003414091 @default.
• W1984432487 cites W2004180909 @default.
• W1984432487 cites W2010655741 @default.
• W1984432487 cites W2023254910 @default.
• W1984432487 cites W2027022352 @default.
• W1984432487 cites W2027532115 @default.
• W1984432487 cites W2029532796 @default.
• W1984432487 cites W2032760352 @default.
• W1984432487 cites W2036975764 @default.
• W1984432487 cites W2040135588 @default.
• W1984432487 cites W2045115038 @default.
• W1984432487 cites W2048842434 @default.
• W1984432487 cites W2052478070 @default.
• W1984432487 cites W2061093127 @default.
• W1984432487 cites W2070831080 @default.
• W1984432487 cites W2071738984 @default.
• W1984432487 cites W2074289058 @default.
• W1984432487 cites W2076024443 @default.
• W1984432487 cites W2091077321 @default.
• W1984432487 cites W2103006990 @default.
• W1984432487 cites W2106483264 @default.
• W1984432487 cites W2124158735 @default.
• W1984432487 cites W2144448362 @default.
• W1984432487 cites W2144792857 @default.
• W1984432487 cites W2156440836 @default.
• W1984432487 cites W2159200136 @default.
• W1984432487 doi "https://doi.org/10.1016/j.biomaterials.2008.10.057" @default.
• W1984432487 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19038441" @default.
• W1984432487 hasPublicationYear "2009" @default.
• W1984432487 type Work @default.
• W1984432487 sameAs 1984432487 @default.
• W1984432487 citedByCount "59" @default.
• W1984432487 countsByYear W19844324872012 @default.
• W1984432487 countsByYear W19844324872013 @default.
• W1984432487 countsByYear W19844324872014 @default.
• W1984432487 countsByYear W19844324872015 @default.
• W1984432487 countsByYear W19844324872016 @default.
• W1984432487 countsByYear W19844324872017 @default.
• W1984432487 countsByYear W19844324872018 @default.
• W1984432487 countsByYear W19844324872019 @default.
• W1984432487 countsByYear W19844324872020 @default.
• W1984432487 countsByYear W19844324872022 @default.
• W1984432487 countsByYear W19844324872023 @default.
• W1984432487 crossrefType "journal-article" @default.
• W1984432487 hasAuthorship W1984432487A5030064573 @default.
• W1984432487 hasAuthorship W1984432487A5079724569 @default.
• W1984432487 hasAuthorship W1984432487A5082819905 @default.
• W1984432487 hasAuthorship W1984432487A5083138002 @default.
• W1984432487 hasAuthorship W1984432487A5087261496 @default.
• W1984432487 hasConcept C104317684 @default.
• W1984432487 hasConcept C111335760 @default.
• W1984432487 hasConcept C12554922 @default.
• W1984432487 hasConcept C129312508 @default.
• W1984432487 hasConcept C135983454 @default.
• W1984432487 hasConcept C14631669 @default.
• W1984432487 hasConcept C1491633281 @default.
• W1984432487 hasConcept C153911025 @default.
• W1984432487 hasConcept C155672457 @default.
• W1984432487 hasConcept C15920480 @default.
• W1984432487 hasConcept C159985019 @default.
• W1984432487 hasConcept C171250308 @default.
• W1984432487 hasConcept C183882617 @default.
• W1984432487 hasConcept C185592680 @default.
• W1984432487 hasConcept C188027245 @default.
• W1984432487 hasConcept C192562407 @default.
• W1984432487 hasConcept C2777366897 @default.
• W1984432487 hasConcept C521977710 @default.
• W1984432487 hasConcept C54009773 @default.
• W1984432487 hasConcept C552990157 @default.
• W1984432487 hasConcept C55493867 @default.
• W1984432487 hasConcept C67407626 @default.
• W1984432487 hasConcept C86803240 @default.
• W1984432487 hasConceptScore W1984432487C104317684 @default.
• W1984432487 hasConceptScore W1984432487C111335760 @default.
• W1984432487 hasConceptScore W1984432487C12554922 @default.
• W1984432487 hasConceptScore W1984432487C129312508 @default.
• W1984432487 hasConceptScore W1984432487C135983454 @default.
• W1984432487 hasConceptScore W1984432487C14631669 @default.
• W1984432487 hasConceptScore W1984432487C1491633281 @default.
• W1984432487 hasConceptScore W1984432487C153911025 @default.
• W1984432487 hasConceptScore W1984432487C155672457 @default.

• next