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• W1984460306 abstract "The aims of this study were to explore the relationship between clinical outcome and HER-2 protein expression in dogs with malignant mammary tumours after surgery and to determine the sequence of the HER-2 gene. Tissues from 91 dogs diagnosed with malignant mammary tumours, six with benign lesions and two normal mammary glands were analysed for the expression of HER-2 by immunohistochemistry (IHC). Exons 14–18 of the HER-2 gene were amplified by reverse transcriptase polymerase chain reaction and sequenced in 19 tumours with IHC scores >2+; larger coding regions of HER-2 were analysed in five tumours. HER-2 protein overexpression was found in 27/91 (29.7%) of canine malignant mammary tumours. Dogs with HER-2-overexpressing malignant mammary tumours tended to have a higher survival rate than those that expressed a normal level of HER-2 within 2 years of surgery. In 3/19 cases, HER-2 sequences had a single nucleotide polymorphism, T1725C, with no change in the amino acid identity (Cys). No other sequence aberration of HER-2 was found." @default.
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• W1984460306 date "2009-04-01" @default.
• W1984460306 modified "2023-10-17" @default.
• W1984460306 title "Increased survival in dogs with malignant mammary tumours overexpressing HER-2 protein and detection of a silent single nucleotide polymorphism in the canine HER-2 gene" @default.
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• W1984460306 doi "https://doi.org/10.1016/j.tvjl.2007.10.013" @default.
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