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- W1984497553 abstract "Liquid-crystal-based biosensors, based on a design by N. L. Abbott [1,2], are drawing increasing attention because of their dramatic optical response to minute changes at their surface, due, for example, to absorption of biomolecules [see figure.] In this work we simplify and improve the precision and reproducibility of such a biosensor. First, we show that the sensor may be used without separately prepared aligning substrate, which makes measurements more reproducible. Second, analyze the birefringence of the sensing liquid crystal with a spectrophotometer, which makes measurements more precise. Third, we observe the optical response and measure spectral output from every individual cell to account for distribution of liquid crystal thickness in the TEM grid, which makes measurement and analysis more consistent. We further study the response of the sensor both on an aqueous solution/air surface and immersed in the solution. These improvements will lead to building less expensive, simpler in operation, and more reliable liquid-crystal-based biosensors. [1] J.M. Brake, M.K. Daschner, Y.-Y. Luk, and N.L. Abbott, Science (New York, N.Y.) 302, 2094 (2003). [2] W. Iglesias, N.L. Abbott, E.K. Mann, and A. Jákli, ACS Applied Materials & Interfaces 4, 6884 (2012)." @default.
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- W1984497553 date "2014-01-01" @default.
- W1984497553 modified "2023-09-26" @default.
- W1984497553 title "Liquid-Crystal-Based Biosensor without Alignment Substrate" @default.
- W1984497553 doi "https://doi.org/10.1016/j.bpj.2013.11.2337" @default.
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