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- W1984651267 abstract "K-ras gene sequences mutant at codon 12 were recovered following differential dot-blot hybridization of genomic DNA from human RSa cells up to 12 days after the cells had been irradiated with far-UV (principally 254 nm). By contrast, no mutant codon 12 sequences were recovered from cells which had been treated with 50 IU/ml human interferon (HuIFN)-α for 24 h prior to their UV exposure. HuIFN-α treatment in combination with anti-HuIFN-α antibody did not lead to the loss of mutant sequences. However, culture of interferon-pretreated cells with medium containing the protease inhibitor antipain (0.01 mM) for 6 h immediately after UV irradiation led to the recovery of mutant codon 12 sequences. Thus, while treatment with HuIFN-α appeared to prevent any UV-induced mutations affecting codon 12 of the K-ras gene from being recovered, the putative antipain-sensitive protease responsible for this suppressive affect appeared to be significantly affected by the protease inhibitor antipain." @default.
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- W1984651267 date "1997-02-01" @default.
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- W1984651267 title "UV-induced mutations affecting codon 12 of the K-ras gene are suppressed by interferon-α in human RSa cells" @default.
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- W1984651267 doi "https://doi.org/10.1016/s0027-5107(96)00204-7" @default.
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