Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984663112> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1984663112 endingPage "960" @default.
- W1984663112 startingPage "951" @default.
- W1984663112 abstract "Intramolecular cross-linking coupled with mass spectrometric identification of cross-linked amino acids is a rapid method for elucidating low-resolution protein tertiary structures or fold families. However, previous cross-linking studies on model proteins, such as cytochrome c and ribonuclease A, identified a limited number of peptide cross-links that are biased toward only a few of the potentially reactive lysine residues. Here, we report an approach to improve the diversity of intramolecular protein cross-linking starting with a systematic quantitation of the reactivity of lysine residues of a model protein, bovine cytochrome c. Relative lysine reactivities among the 18 lysine residues of cytochrome c were determined by the ratio of d0 and acetyl-d3 groups at each lysine after partial acetylation with sulfosuccinimidyl acetate followed by denaturation and quantitative acetylation of remaining unmodified lysines with acetic-d6 anhydride. These lysine reactivities were then compared with theoretically derived pKa and relative solvent accessibility surface values. To ascertain if partial N-acetylation of the most reactive lysine residues prior to cross-linking can redirect and increase the observable Lys-Lys cross-links, partially acetylated bovine cytochrome c was cross-linked with the amine-specific, bis-functional reagent, bis(sulfosuccinimidyl)suberate. After proteolysis and mass spectrometry analysis, partial acetylation was shown to significantly increase the number of observable peptides containing Lys-Lys cross-links, shifting the pattern from the most reactive lysine residues to less reactive ones. More importantly, these additional cross-linked peptides contained novel Lys-Lys cross-link information not seen in the non-acetylated protein and provided additional distance constraints that were consistent with the crystal structure and facilitated the identification of the proper protein fold." @default.
- W1984663112 created "2016-06-24" @default.
- W1984663112 creator A5003883020 @default.
- W1984663112 creator A5020955332 @default.
- W1984663112 creator A5021662454 @default.
- W1984663112 creator A5037988546 @default.
- W1984663112 creator A5052097118 @default.
- W1984663112 creator A5056510065 @default.
- W1984663112 creator A5059465522 @default.
- W1984663112 creator A5068729397 @default.
- W1984663112 creator A5084313185 @default.
- W1984663112 date "2008-01-18" @default.
- W1984663112 modified "2023-09-22" @default.
- W1984663112 title "Partial Acetylation of Lysine Residues Improves Intraprotein Cross-Linking" @default.
- W1984663112 cites W1974843618 @default.
- W1984663112 cites W1981593008 @default.
- W1984663112 cites W1985219728 @default.
- W1984663112 cites W1995050941 @default.
- W1984663112 cites W2012534919 @default.
- W1984663112 cites W2012601398 @default.
- W1984663112 cites W2021534482 @default.
- W1984663112 cites W2023463682 @default.
- W1984663112 cites W2027188202 @default.
- W1984663112 cites W2029318495 @default.
- W1984663112 cites W2031513106 @default.
- W1984663112 cites W2070247089 @default.
- W1984663112 cites W2073035613 @default.
- W1984663112 cites W2110397834 @default.
- W1984663112 cites W2114812482 @default.
- W1984663112 cites W2168211076 @default.
- W1984663112 doi "https://doi.org/10.1021/ac701636w" @default.
- W1984663112 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18201069" @default.
- W1984663112 hasPublicationYear "2008" @default.
- W1984663112 type Work @default.
- W1984663112 sameAs 1984663112 @default.
- W1984663112 citedByCount "35" @default.
- W1984663112 countsByYear W19846631122012 @default.
- W1984663112 countsByYear W19846631122013 @default.
- W1984663112 countsByYear W19846631122014 @default.
- W1984663112 countsByYear W19846631122015 @default.
- W1984663112 countsByYear W19846631122016 @default.
- W1984663112 countsByYear W19846631122017 @default.
- W1984663112 countsByYear W19846631122018 @default.
- W1984663112 countsByYear W19846631122019 @default.
- W1984663112 countsByYear W19846631122020 @default.
- W1984663112 crossrefType "journal-article" @default.
- W1984663112 hasAuthorship W1984663112A5003883020 @default.
- W1984663112 hasAuthorship W1984663112A5020955332 @default.
- W1984663112 hasAuthorship W1984663112A5021662454 @default.
- W1984663112 hasAuthorship W1984663112A5037988546 @default.
- W1984663112 hasAuthorship W1984663112A5052097118 @default.
- W1984663112 hasAuthorship W1984663112A5056510065 @default.
- W1984663112 hasAuthorship W1984663112A5059465522 @default.
- W1984663112 hasAuthorship W1984663112A5068729397 @default.
- W1984663112 hasAuthorship W1984663112A5084313185 @default.
- W1984663112 hasConcept C104317684 @default.
- W1984663112 hasConcept C119157956 @default.
- W1984663112 hasConcept C161790260 @default.
- W1984663112 hasConcept C185592680 @default.
- W1984663112 hasConcept C2776016237 @default.
- W1984663112 hasConcept C2779281246 @default.
- W1984663112 hasConcept C2779854550 @default.
- W1984663112 hasConcept C515207424 @default.
- W1984663112 hasConcept C55493867 @default.
- W1984663112 hasConcept C71240020 @default.
- W1984663112 hasConceptScore W1984663112C104317684 @default.
- W1984663112 hasConceptScore W1984663112C119157956 @default.
- W1984663112 hasConceptScore W1984663112C161790260 @default.
- W1984663112 hasConceptScore W1984663112C185592680 @default.
- W1984663112 hasConceptScore W1984663112C2776016237 @default.
- W1984663112 hasConceptScore W1984663112C2779281246 @default.
- W1984663112 hasConceptScore W1984663112C2779854550 @default.
- W1984663112 hasConceptScore W1984663112C515207424 @default.
- W1984663112 hasConceptScore W1984663112C55493867 @default.
- W1984663112 hasConceptScore W1984663112C71240020 @default.
- W1984663112 hasIssue "4" @default.
- W1984663112 hasLocation W19846631121 @default.
- W1984663112 hasLocation W19846631122 @default.
- W1984663112 hasOpenAccess W1984663112 @default.
- W1984663112 hasPrimaryLocation W19846631121 @default.
- W1984663112 hasRelatedWork W1968000388 @default.
- W1984663112 hasRelatedWork W1985236672 @default.
- W1984663112 hasRelatedWork W2021475144 @default.
- W1984663112 hasRelatedWork W2327853242 @default.
- W1984663112 hasRelatedWork W2561111032 @default.
- W1984663112 hasRelatedWork W3199543258 @default.
- W1984663112 hasRelatedWork W3201347765 @default.
- W1984663112 hasRelatedWork W4230421056 @default.
- W1984663112 hasRelatedWork W4243373834 @default.
- W1984663112 hasRelatedWork W68444996 @default.
- W1984663112 hasVolume "80" @default.
- W1984663112 isParatext "false" @default.
- W1984663112 isRetracted "false" @default.
- W1984663112 magId "1984663112" @default.
- W1984663112 workType "article" @default.