FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984710010> ?p ?o ?g. }

• W1984710010 endingPage "694" @default.
• W1984710010 startingPage "688" @default.
• W1984710010 abstract "The rate at which equine and macaque ovarian tissue sections are first cooled from +25°Cto+4°C has a significant effect on the measured water transport when the tissues are subsequently frozen in 0.85M solutions of glycerol, dimethylsulfoxide (DMSO), or ethylene glycol (EG). To determine whether the response of ovarian tissues is altered if they are suspended in mixtures of cryoprotective agents (CPAs), rather than in solutions of a single CPA, we have now measured the subzero water transport from ovarian tissues that were suspended in mixtures of DMSO and EG. Sections of freshly collected equine and macaque ovaries were suspended either in a mixture of 0.9M EG plus 0.7M DMSO (equivalent to a mixture of ∼5%v∕v of EG and DMSO) or in a 1.6M solution of only DMSO or only EG. The tissue sections were cooled from +25°Cto+4°C and then frozen to subzero temperatures at 5°C∕min. As the tissues were being frozen, a shape-independent differential scanning calorimeter technique was used to measure water loss from the tissues and, consequently, the best fit membrane permeability parameters (Lpg and ELp) of ovarian tissues during freezing. In the mixture of DMSO+EG, the respective values of Lpg and ELp for equine tissue first cooled at 40°C∕min between +25°C and +4°C before being frozen were 0.15μm∕minatm and 7.6kcal∕mole. The corresponding Lpg and ELp values for equine tissue suspended in 1.6M DMSO were 0.12μm∕minatm and 27.2kcal∕mole; in 1.6M EG, the values were 0.06μm∕minatm and 21.9kcal∕mole, respectively. For macaque ovarian tissues suspended in the mixture of DMSO+EG, the respective values of Lpg and ELp were 0.26μm∕minatm and 26.2kcal∕mole. Similarly, the corresponding LLg and ELp values for macaque tissue suspended in 1.6M DMSO were 0.22μm∕minatm and 31.4kcal∕mole; in 1.6M EG, the values were 0.20μm∕minatm and 27.9kcal∕mole. The parameters for both equine and macaque tissue samples suspended in the DMSO+EG mixture and first cooled at 0.5°C∕min between +25°C and +4°C were very similar to the corresponding values for samples cooled at 40°C∕min. In contrast, the membrane parameters of equine and macaque samples first cooled at 0.5°C∕min in single-component solutions were significantly different from the corresponding values for samples cooled at 40°C∕min. These results show that the membrane properties of ovarian cells from two species are different, and that the membrane properties are significantly affected both by the solution in which the tissue is suspended and by the rate at which the tissue is cooled from +25°Cto+4°C before being frozen. These observations suggest that these variables ought to be considered in the derivation of methods to cryopreserve ovarian tissues." @default.
• W1984710010 created "2016-06-24" @default.
• W1984710010 creator A5019333768 @default.
• W1984710010 creator A5038814570 @default.
• W1984710010 creator A5071240649 @default.
• W1984710010 date "2007-02-14" @default.
• W1984710010 modified "2023-09-26" @default.
• W1984710010 title "Membrane Transport Properties of Equine and Macaque Ovarian Tissues Frozen in Mixtures of Dimethylsulfoxide and Ethylene Glycol" @default.
• W1984710010 cites W1951167878 @default.
• W1984710010 cites W1984271534 @default.
• W1984710010 cites W1988355952 @default.
• W1984710010 cites W1996567327 @default.
• W1984710010 cites W2000003753 @default.
• W1984710010 cites W2000691316 @default.
• W1984710010 cites W2012689102 @default.
• W1984710010 cites W2013230763 @default.
• W1984710010 cites W2032414770 @default.
• W1984710010 cites W2041879182 @default.
• W1984710010 cites W2050842249 @default.
• W1984710010 cites W2052389135 @default.
• W1984710010 cites W2055291801 @default.
• W1984710010 cites W2057826893 @default.
• W1984710010 cites W2064320023 @default.
• W1984710010 cites W2068468196 @default.
• W1984710010 cites W2077055926 @default.
• W1984710010 cites W2085150454 @default.
• W1984710010 cites W2085580207 @default.
• W1984710010 cites W2089086212 @default.
• W1984710010 cites W2092253687 @default.
• W1984710010 cites W2095617885 @default.
• W1984710010 cites W2106428948 @default.
• W1984710010 cites W2107618142 @default.
• W1984710010 cites W2109678032 @default.
• W1984710010 cites W2110232055 @default.
• W1984710010 cites W2113060660 @default.
• W1984710010 cites W2121438091 @default.
• W1984710010 cites W2130833606 @default.
• W1984710010 cites W2143990115 @default.
• W1984710010 cites W2144436656 @default.
• W1984710010 cites W2148709728 @default.
• W1984710010 cites W2153810688 @default.
• W1984710010 cites W2153820217 @default.
• W1984710010 cites W2155255557 @default.
• W1984710010 cites W2160650528 @default.
• W1984710010 cites W2162137928 @default.
• W1984710010 cites W2164159365 @default.
• W1984710010 cites W2166478627 @default.
• W1984710010 cites W2171866935 @default.
• W1984710010 cites W4244065966 @default.
• W1984710010 cites W4249251024 @default.
• W1984710010 cites W565817459 @default.
• W1984710010 doi "https://doi.org/10.1115/1.2768107" @default.
• W1984710010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17887894" @default.
• W1984710010 hasPublicationYear "2007" @default.
• W1984710010 type Work @default.
• W1984710010 sameAs 1984710010 @default.
• W1984710010 citedByCount "16" @default.
• W1984710010 countsByYear W19847100102012 @default.
• W1984710010 countsByYear W19847100102014 @default.
• W1984710010 countsByYear W19847100102016 @default.
• W1984710010 countsByYear W19847100102017 @default.
• W1984710010 countsByYear W19847100102018 @default.
• W1984710010 countsByYear W19847100102019 @default.
• W1984710010 countsByYear W19847100102021 @default.
• W1984710010 countsByYear W19847100102022 @default.
• W1984710010 crossrefType "journal-article" @default.
• W1984710010 hasAuthorship W1984710010A5019333768 @default.
• W1984710010 hasAuthorship W1984710010A5038814570 @default.
• W1984710010 hasAuthorship W1984710010A5071240649 @default.
• W1984710010 hasConcept C134018914 @default.
• W1984710010 hasConcept C13965031 @default.
• W1984710010 hasConcept C178790620 @default.
• W1984710010 hasConcept C185592680 @default.
• W1984710010 hasConcept C2777516009 @default.
• W1984710010 hasConcept C2778324911 @default.
• W1984710010 hasConcept C2778452553 @default.
• W1984710010 hasConcept C2780881558 @default.
• W1984710010 hasConcept C41625074 @default.
• W1984710010 hasConcept C43617362 @default.
• W1984710010 hasConcept C55493867 @default.
• W1984710010 hasConcept C86803240 @default.
• W1984710010 hasConceptScore W1984710010C134018914 @default.
• W1984710010 hasConceptScore W1984710010C13965031 @default.
• W1984710010 hasConceptScore W1984710010C178790620 @default.
• W1984710010 hasConceptScore W1984710010C185592680 @default.
• W1984710010 hasConceptScore W1984710010C2777516009 @default.
• W1984710010 hasConceptScore W1984710010C2778324911 @default.
• W1984710010 hasConceptScore W1984710010C2778452553 @default.
• W1984710010 hasConceptScore W1984710010C2780881558 @default.
• W1984710010 hasConceptScore W1984710010C41625074 @default.
• W1984710010 hasConceptScore W1984710010C43617362 @default.
• W1984710010 hasConceptScore W1984710010C55493867 @default.
• W1984710010 hasConceptScore W1984710010C86803240 @default.
• W1984710010 hasIssue "5" @default.
• W1984710010 hasLocation W19847100101 @default.
• W1984710010 hasLocation W19847100102 @default.
• W1984710010 hasOpenAccess W1984710010 @default.
• W1984710010 hasPrimaryLocation W19847100101 @default.

• next