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- W1984738861 abstract "A new thiol weak-fluorescence probe, 5-maleimidyl-2-(m-methylphenyl)benzoxazole (MMPB), gives a highly fluorescence product in the presence of Cys. In this paper, MMPB has been developed for the fluorimetric determination of cysteine (Cys). At lambda(ex)/lambda(em) = 305.6/425.6 nm, the linear range is from 0 to 3.3 x 10(-7) mol l(-1) and the detection limit (sigma = 3) of 6.2 x 10(-10) mol l(-1). The main advantage of this method lies in the relative high selectivity compared with the methods using other N-substituted maleimide type of thiol reagents, in which 0.15-fold (molar ratio) of GSH is allowed and most of other amino acids at 100-fold (molar ratio) level had no obvious effect on the results. The proposed method has been applied to the determination of Cys in real samples." @default.
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- W1984738861 date "2002-10-01" @default.
- W1984738861 modified "2023-09-27" @default.
- W1984738861 title "Spectrofluorimetric determination of cysteine by 5-maleimidyl-2-(m-methylphenyl)benzoxazole" @default.
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- W1984738861 doi "https://doi.org/10.1016/s1386-1425(02)00003-3" @default.
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