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• W1984761310 abstract "You have accessJournal of UrologyKidney Cancer: Basic Research I1 Apr 2014MP23-13 MICRO-RNA DIFFERENTIATES TUMOR TYPE & METASTATIC POTENTIAL IN RCC Michael Garcia-Roig, Nicolas Ortiz, and Vinata Lokeshwar Michael Garcia-RoigMichael Garcia-Roig More articles by this author , Nicolas OrtizNicolas Ortiz More articles by this author , and Vinata LokeshwarVinata Lokeshwar More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.881AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Small renal tumors (<4 cm) pose a challenge as 16-28% are benign, indistinguishable from malignant lesions on imaging & pose a metastatic risk. Percutaneous biopsy has regained popularity for small masses by using histology, immunology or molecular markers for diagnosis and prognosis. miRNAs are 22-nucleotide noncoding segments regulating gene expression by causing degradation of target mRNAs. We examined the differential expression of microRNAs (miRs) in renal cell carcinoma (RCC) & normal kidney specimens. Our objective was to examine whether miRNA expression can identify tumor type & predict metastasis. METHODS RCC & normal kidney specimens were collected from 86 patients at the time of nephrectomy. miR enriched total RNA was isolated from 46 RCC & 59 normal specimens. Clinical & pathologic data were collected including histology (oncocytoma 6, chromophobe 4, papillary 6, clear cell 25, sarcoma 4, & collecting duct 1), stage (T1a, 11; T1b, 9; T2, 9; T3, 10; T4, 1), renal vein involvement (+: 5; −: 41), & lymph node status (+: 4; −: 41). At 14 months median follow−up, 6 patients were positive for metastasis. Microarray (Thermo Fisher Scientific 10.1) was performed on 30 samples to characterize 554 miRNA targets. 7 miRs were chosen (mir−21, 150, 155, 142−3p, 142−5p, 192, & 194) based on the fold change in tumor samples. Their expression was quantified by quantitative PCR, & normalized to a housekeeping miR. Statistical analysis was performed using the Mann−Whiteny U−test & multivariate analysis. RESULTS miR microarray of RCC vs non-RCC specimens identified significant upregulation of 65 (mean fold change (FC) 2.13), & downregulation of 47 (mean FC 1.7) miRNAs in (p<0.05), respectively. Q-PCR of RCC specimens showed differential expression of miRs-21, 155, 192 & 194. miR-150 & miR-155 levels were 5.8-8.2-fold upregulated in chromophobe (25.8±21; 2.3±2.9) vs. oncocytomas (4.4±2.0; 0.28±0.36; P=0.038). miR-21, miR-1423P, miR-1425P & miR-155 were 3-10-fold upregulated in CC-RCC (1731±1145; 77±174; 4.2±5.2; 2.2±2.7) vs oncocytoma (553±523; 7.3±8.2; 0.43±0.42; 0.28±0.36), respectively (P ≤0.01). Only miR-155 was 5.5-fold upregulated in papillary tumors vs oncocytoma (P=0.015). In multivariate analysis including TNM stage, renal vein invasion, age, gender & miR levels, only mir−194 expression correlated with metastasis (p=0.034; Xˆ2 = 4.5). CONCLUSIONS miR−155, 192, 194, & 21 are differentially expressed in kidney cancer. miR−194 expression independently associates with metastasis. miR-155 was differentially expressed in all RCC types when compared to normal kidney & oncocytomas. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e248 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Michael Garcia-Roig More articles by this author Nicolas Ortiz More articles by this author Vinata Lokeshwar More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ..." @default.
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• W1984761310 title "MP23-13 MICRO-RNA DIFFERENTIATES TUMOR TYPE & METASTATIC POTENTIAL IN RCC" @default.
• W1984761310 doi "https://doi.org/10.1016/j.juro.2014.02.881" @default.
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