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- W1984767413 abstract "A flow-injection method for the determination of serine using a mini-column containing immobilized serine dehydratase isolated and purified from rat liver is described. Ammonia produced from the enzymatic reaction is coupled with hypochlorite and phenol in an alkaline medium yields a blue product due to the indophenol anion formation, which is the basis of a spectrophotometric detection at 640 nm. The limit of detection (2×blank noise) is 0.01 mM with a sample throughput of 25 h−1. Calibration graph is linear in the range 0.2–1.0 mM, with relative standard deviation 0.6–1.0%." @default.
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- W1984767413 date "1967-08-01" @default.
- W1984767413 modified "2023-10-11" @default.
- W1984767413 title "Crystallization and characteristics of serine dehydratase from rat liver" @default.
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- W1984767413 doi "https://doi.org/10.1016/0006-291x(67)90318-x" @default.
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