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- W1984865400 abstract "To identify human sperm proteins involved in homologous zona pellucida (ZP) interaction.Prospective study.Basic research laboratory.Semen samples from normozoospermic donors, tissue sections from surgical pieces, and ZP from nonfertilized oocytes.Antibodies for sperm proteins (HSE; high salt extract) were developed (anti-HSE) and partially characterized. Participation of sperm proteins on ZP-interaction was tested with the hemizona assay (HZA). Antigens were immunolocalized in sperm and tissues.Sperm and tissue immunostaining; Western blotting; and number of sperm bound to the ZP.Anti-HSE antibodies recognized several polypeptides in HSE (9 to 200 kd). Specific antibodies for 49 and 66 kd proteins (p49, p66) were obtained. Both (anti-p49 and anti-p66) stained the head of ejaculated and capacitated sperm. In the HZA, sperm preincubation with a mixture of anti-p49 and anti-p66 (100 micro g/mL) resulted in a decrease in the number of spermatozoa bound to the ZP. Presence of p66 (10 micro g/mL) inhibited sperm-ZP interaction. In contrast, p49 did not alter sperm binding to the ZP. Immunohistochemical analysis showed that p66 is present in the epididymis. No staining was observed in testicular sections.We found that p66 is an epididymal protein that participates in human sperm interaction with homologous ZP." @default.
- W1984865400 created "2016-06-24" @default.
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- W1984865400 date "2003-06-01" @default.
- W1984865400 modified "2023-09-28" @default.
- W1984865400 title "Identification of human sperm proteins involved in the interaction with homologous zona pellucida" @default.
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- W1984865400 doi "https://doi.org/10.1016/s0015-0282(03)00251-6" @default.
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